A sensitive HPLC method for the assessment of metabolic conversion of estrogens

B. Delvoux, Bert Delvoux, Delvoux B, Husen B, Bettina Husen, B Husen, Aldenhoff Y, Y. Aldenhoff, Koole L, L Koole, Léo H. Koole, G.A.J. Dunselman, Dunselman G, G Dunselman, H Thole, Thole H, H. Thöle, Groothuis P, Patrick G. Groothuis, P Groothuis
The Journal of steroid biochemistry and molecular biology · 2007 · vol. 104(3-5) , pp. 246–251 · doi:10.1016/j.jsbmb.2007.03.006 · PMID:17481886 · W2085222215
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This paper describes a sensitive high-performance liquid chromatography (HPLC) method for measuring the metabolic conversion of estrogens.

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Abstract

Disorders of estrogen-responsive tissues are frequently associated with aberrations in steroid metabolism due to altered expression of synthesizing and metabolizing enzymes. For instance, overexposure to unopposed 17beta-estradiol has been associated with the pathogenesis of endometrial proliferative disorders, such as endometriosis. Investigations into the metabolic conversion in tissues and cells have been rather limited. This is mostly due to fact that such studies have to make use of radioactive steroid hormones and expensive equipment to obtain sufficient sensitivity. We adapted a sensitive non-radioactive HPLC method to study estrogen metabolism in more detail. This HPLC method is based on the solid phase extraction of estrogens and the derivatization of the steroids with 2-(4-carboxy-phenyl)-5,6-dimethylbenzimidazole. The technique is sensitive, robust and is useful for the detection of aromatase, 17beta-HSD types 1 and 2 and sulfatase activities in lysates of placenta and endometrium.

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MeSH descriptors

Chromatography, High Pressure Liquid Estrogens Estrogens 17-Hydroxysteroid Dehydrogenases 17-Hydroxysteroid Dehydrogenases Aromatase Aromatase Chromatography, High Pressure Liquid Endometrium Endometrium Endometrium Estrogens Female Humans Models, Biological Pilot Projects Placenta Placenta Placenta Sensitivity and Specificity

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