Hysterosalpingography contrast media and chromotubation dye inhibit peritoneal lymphocyte and macrophage function in vitro: a potential mechanism for fertility enhancement

Sandra B. Goodman; S B Goodman; Mitchel S. Rein; M S Rein; J A Hill; Joseph A. Hill

doi:10.1016/s0015-0282(16)55922-6

Hysterosalpingography contrast media and chromotubation dye inhibit peritoneal lymphocyte and macrophage function in vitro: a potential mechanism for fertility enhancement

Sandra B. Goodman, S B Goodman, Mitchel S. Rein, M S Rein, J A Hill, Joseph A. Hill

Fertility and sterility · 1993 · vol. 59(5) , pp. 1022–1027 · doi:10.1016/s0015-0282(16)55922-6 · PMID:8387432 · W93909586

Lab / animal OA: closed CC0 ⤵ 6 in-corpus citations

🔓 Open OA copy View on OpenAlex View on PubMed View at publisher

⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-12 ⓘ

This in vitro study found that hysterosalpingography contrast media and chromotubation dyes negatively impact peritoneal lymphocyte and macrophage function.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

OBJECTIVE: To determine the effects of hysterosalpingography (HSG) contrast media (CM) and chromotubation dye on peritoneal lymphocyte proliferation and macrophage phagocytosis in vitro. DESIGN: Peritoneal fluid (PF) lymphocytes and macrophages were isolated from 40 subfertile women undergoing diagnostic laparoscopy and 12 fertile women having laparoscopic tubal ligation. Dilutions of renografin, ethiodol, methylene blue, and indigo carmine were added to peritoneal lymphocyte and macrophage cultures. Tissue culture media alone served as control. Lymphocyte proliferation was assessed by hemocytometer counts and 3H-thymidine incorporation. Macrophage function was determined by phagocytosis of fluorescent microspheres. RESULTS: Peritoneal lymphocyte proliferation and macrophage phagocytosis were significantly inhibited by renografin, ethiodol, methylene blue, and indigo carmine in a dose-dependent manner. CONCLUSION: Inhibition of PF immune cell function by HSG CM and chromotubation dye may provide a potential mechanism for fertility enhancement after these diagnostic procedures.

My notes (saved in your browser only)

MeSH descriptors

Coloring Agents Contrast Media Hysterosalpingography Lymphocyte Activation Macrophages Sterilization, Tubal T-Lymphocytes Cells, Cultured Cell Survival Cell Survival Coloring Agents Contrast Media Diatrizoate Meglumine Diatrizoate Meglumine Ethiodized Oil Ethiodized Oil Female Humans Indigo Carmine Indigo Carmine

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (25)

Altered maturation and function of peritoneal macrophages: possible role in pathogenesis of endometriosis via openalex
Characterization of leukocyte subpopulations in the peritoneal fluid of women with endometriosis via openalex
Ethiodol oil contrast medium inhibits macrophage phagocytosis and adherence by altering membrane electronegativity and microviscosity via openalex
Experimental evidence for failure to implant as a mechanism of infertility associated with endometriosis via openalex
Peritoneal fluid in patients with and without endometriosis: prostanoids and macrophages and their effect on the spermatozoa penetration assay via openalex
Release of tumor necrosis factor-alpha by human peritoneal macrophages in vivo and in vitro via openalex
doi:10.1016/s0015-0282(16)59056-6 via openalex
doi:10.1095/biolreprod44.1.69 via openalex
W49840345 via openalex
doi:10.1016/0002-9378(88)90810-1 via openalex
doi:10.1016/s0015-0282(16)60185-1 via openalex
doi:10.1016/s0015-0282(16)48987-9 via openalex
doi:10.1016/s0015-0282(16)45567-6 via openalex
doi:10.1016/s0015-0282(16)30725-7 via openalex
doi:10.1016/s0015-0282(16)49657-3 via openalex
doi:10.1016/0002-9378(49)90378-6 via openalex
doi:10.1016/0002-9378(89)90179-8 via openalex
doi:10.1016/s0015-0282(16)53158-6 via openalex
doi:10.1016/s0015-0282(16)33788-8 via openalex
doi:10.1097/00004424-198809001-00038 via openalex
doi:10.1016/s0015-0282(16)47417-0 via openalex
doi:10.4049/jimmunol.139.7.2250 via openalex
doi:10.1016/0028-2243(88)90060-3 via openalex
doi:10.1016/s0015-0282(16)38404-7 via openalex
doi:10.1016/s0015-0282(16)44658-3 via openalex

Cited by (6)

Source provenance

openalex: last seen: 2026-05-14T06:16:54.145842+00:00
pubmed: last seen: 2026-10-08T21:59:31.578486+00:00
unpaywall: last seen: 2026-09-17T06:32:46.214484+00:00

License: CC0 · commercial use OK

[1] Altered maturation and function of peritoneal macrophages: possible role in pathogenesis of endometriosis via openalex

[2] Characterization of leukocyte subpopulations in the peritoneal fluid of women with endometriosis via openalex

[3] Ethiodol oil contrast medium inhibits macrophage phagocytosis and adherence by altering membrane electronegativity and microviscosity via openalex

[4] Experimental evidence for failure to implant as a mechanism of infertility associated with endometriosis via openalex

[5] Peritoneal fluid in patients with and without endometriosis: prostanoids and macrophages and their effect on the spermatozoa penetration assay via openalex

[6] Release of tumor necrosis factor-alpha by human peritoneal macrophages in vivo and in vitro via openalex

[7] doi:10.1016/s0015-0282(16)59056-6 via openalex

[8] doi:10.1095/biolreprod44.1.69 via openalex

[9] W49840345 via openalex

[10] doi:10.1016/0002-9378(88)90810-1 via openalex

[11] doi:10.1016/s0015-0282(16)60185-1 via openalex

[12] doi:10.1016/s0015-0282(16)48987-9 via openalex

[13] doi:10.1016/s0015-0282(16)45567-6 via openalex

[14] doi:10.1016/s0015-0282(16)30725-7 via openalex

[15] doi:10.1016/s0015-0282(16)49657-3 via openalex

[16] doi:10.1016/0002-9378(49)90378-6 via openalex

[17] doi:10.1016/0002-9378(89)90179-8 via openalex

[18] doi:10.1016/s0015-0282(16)53158-6 via openalex

[19] doi:10.1016/s0015-0282(16)33788-8 via openalex

[20] doi:10.1097/00004424-198809001-00038 via openalex

[21] doi:10.1016/s0015-0282(16)47417-0 via openalex

[22] doi:10.4049/jimmunol.139.7.2250 via openalex

[23] doi:10.1016/0028-2243(88)90060-3 via openalex

[24] doi:10.1016/s0015-0282(16)38404-7 via openalex

[25] doi:10.1016/s0015-0282(16)44658-3 via openalex