Endoplasmic reticulum stress of endometrial mesenchymal stem cells in endometriosis

Selenay Furat Rençber, Rencber SF, Selenay Furat Rencber, Yazır Y, Yusufhan Yazır, Mehmet Sarıhan, Sarıhan M, Sezer Z, Zehra Sezer, Zeynep Ece Utkan Korun, Korun ZEU, Ahmet Ozturk, Ahmet Öztürk, Ozturk A, Gökhan Duruksu, Gokhan Duruksu, Guzel E, Elif Güzel, Elif Guzel, Gürler Akpınar, Gurler Akpınar, Akpınar G, Aydın Corakci, Corakci A, Aydın Çorakçı
Tissue & cell · 2024 · vol. 91 , pp. 102544 · doi:10.1016/j.tice.2024.102544 · PMID:39217786 · W4401946439
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⚙ AI-generated summary by gemini-2.5-flash-lite+body, 2026-06-13 ⓘ

This study examined endoplasmic reticulum stress in endometrial mesenchymal stem cells from women with and without endometriosis, finding distinct ER stress protein levels and ultrastructural evidence.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-13 · read from full text ⓘ

This study examined endoplasmic reticulum (ER) stress in endometrial mesenchymal stem cells (eMSCs) isolated from women with endometriosis and from healthy subjects across proliferative and secretory phases. Using proteomic profiling of ER stress-related proteins with validation by Western blot and immunofluorescence, the authors identified significantly different levels of DDRGK1, RTN3, ERp44, TMED2, TMEM33, and TMX3 in endometriosis groups, supported by RT-qPCR gene expression results. TEM ultrastructural analysis further showed evidence of ER stress in eMSCs from endometriosis samples, and ER stress markers were detected in SUSD2+ eMSCs via confocal microscopy. The paper does not explicitly state limitations in the provided text. This paper is centrally about endometriosis — it focuses on ER stress in eMSCs as part of endometriosis pathogenesis.

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Abstract

OBJECTIVE: The human endometrium has significant regenerative abilities due to stem cells, which are vital in immunomodulation, immune tolerance, steroid hormone response, and inflammation. Endometriosis, an inflammatory gynecological disorder where endometrium-like tissue grows outside uterus, affects millions of women and often causes infertility. Recent research indicates that stem cells contribute to pathology of endometriosis. ER stress is implicated in various diseases, including endometriosis. This study aims to examine ER stress in eMSCs within endometriosis pathogenesis and uncover underlying disease mechanisms. METHODS: Samples were collected from healthy subjects and women with endometriosis in both proliferative and secretory phases. eMSCs were isolated and characterized via flow cytometry. ER stress protein levels were assessed using proteomic analysis, with validation through Western Blot and immunofluorescence staining. Gene expression was analyzed by RT-qPCR, and ultrastructural examination of eMSCs was conducted using TEM. ER stress markers in tissue samples were detected in SUSD2+ eMSCs through immunofluorescence staining and visualized using a confocal microscope. Statistical analysis was performed using SPSS program. RESULTS: The proteomics analysis uncovered ER stress-related proteins (DDRGK1, RTN3, ERp44, TMED2, TMEM33, TMX3) whose levels were significantly distinct from control group. Western Blot analysis and immunofluorescence staining results at protein level; RT-qPCR results at gene level supported these findings. TEM analysis also showed ultrastructural presence of ER stress in endometriosis groups. CONCLUSION: Presence of ER stress in eMSCs in pathogenesis of endometriosis has been demonstrated using various methods. Our research has potential to shed light on pathology of endometriosis and offer promising avenues for non-invasive diagnosis and potential treatment.
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Keywords

Apoptosis, Autophagy, Endometriosis, Endoplasmic reticulum stress, Infertility, Mesenchymal stem cells - Open Archive Collection: AVESIS Open Access Collection - Kocaeli University Affiliated: Yes

Abstract

Objective: The human endometrium has significant regenerative abilities due to stem cells, which are vital in immunomodulation, immune tolerance, steroid hormone response, and inflammation. Endometriosis, an inflammatory gynecological disorder where endometrium-like tissue grows outside uterus, affects millions of women and often causes infertility. Recent research indicates that stem cells contribute to pathology of endometriosis. ER stress is implicated in various diseases, including endometriosis. This study aims to examine ER stress in eMSCs within endometriosis pathogenesis and uncover underlying disease mechanisms. Methods: Samples were collected from healthy subjects and women with endometriosis in both proliferative and secretory phases. eMSCs were isolated and characterized via flow cytometry. ER stress protein levels were assessed using proteomic analysis, with validation through Western Blot and immunofluorescence staining. Gene expression was analyzed by RT-qPCR, and ultrastructural examination of eMSCs was conducted using TEM. ER stress markers in tissue samples were detected in SUSD2+ eMSCs through immunofluorescence staining and visualized using a confocal microscope. Statistical analysis was performed using SPSS program. Results: The proteomics analysis uncovered ER stress-related proteins (DDRGK1, RTN3, ERp44, TMED2, TMEM33, TMX3) whose levels were significantly distinct from control group. Western Blot analysis and immunofluorescence staining results at protein level; RT-qPCR results at gene level supported these findings. TEM analysis also showed ultrastructural presence of ER stress in endometriosis groups. Conclusion: Presence of ER stress in eMSCs in pathogenesis of endometriosis has been demonstrated using various methods. Our research has potential to shed light on pathology of endometriosis and offer promising avenues for non-invasive diagnosis and potential treatment.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (65)

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