Effect of bone marrow mesenchymal stem cells transplantation on BMP-15 expression and Graafian follicle count in mice model of endometriosis

To determine the effect of bone marrow mesenchy-mal stem cells (BMSCs) on BMP-15 expression and Graafian follicle count in endometriosis mice.

This study was a laboratory randomized clinical trial on Mus musculus. The object of the study was 42 mice which were divided into 3 groups, the control, endome-triosis, and endometriosis + BMSCs groups. Comparison of BMP-15 expression and Graafian follicle count between groups was evaluated.

Immunohistochemical analysis showed that BMP-15 expression in control, endometriosis, and endometriosis + BMSCs groups had p=0.551, p=0.446 and p=0.917 with ANOVA test p=0.273, indicating no statistically significant differences between groups . Graafian follicular count in the three groups had p=0.31, p=0.001, and p=0.006, with the Kruskal-Wallis test p=0.001. Graafian follicles in the endometriosis + BMSCs group were higher than those in control and endometriosis groups. Conclusion:In the endometriosis mouse model with bone mar-row stem cell transplantation the BMP-15 expression in each group did not show a difference, but a significant difference was found in the number of Graafian follicles. Xu, B., Guo, N., Shi, W., et al. 2015. Oocyte quality is decreased in women with minimal or mild endometriosis. Persani, L., Rosetti, R., Di Pasquale, E., et al. ,2014. The fundamental role of bone morphogenetic protein 15 in ovarian function and its involvement in female fertility disorders. 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Recent Arguments Against Germ Cell Renewal in the Adult Human Ovary: Is an Absence of Marker Gene Expression Really Acceptable Evidence of an Absence of Oogenesis? Cell Cycle . Volume 6, 2007 - Issue 8 Hendarto, H., Komarhadi, M., Darmawanti, E. & Widjiati, S.,2013. The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat. J Stem Cell Res Ther, 3, 2. Santoso, B., Sulistiyono, A. (2014). Efektivitas terapi Rat Bone Marrow Stem Cell pada tikus Model Sindroma Ovarium Poli Kistik Terhadap Folikulogenesis, Ekspresi Transforming Growth Faktor B. Penelitian unggulan perguruan tinggi Universitas Airlangga (2014). 1. Copyright of the article is transferred to the journal, by the knowledge of the author, whilst the moral right of the publication belongs to the author. 2. 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