iScience

Studying human development presents significant ethical and technical challenges. Yet, the integration of stem cell technology and engineering tools has provided unprecedented insights into early lineage specification and the morphogenetic …

Uterine dendritic cells (uDCs) are critical for endometrial function, yet their origin and functional roles during the pre- and post-implantation periods in the human endometrium remain largely unknown. We hypothesize that distinct uDC subs…

We recently showed that endometriotic peritoneal fluid (PF) altered the regulation of enhancer of zeste homolog 2 (EZH2) and H3K27me3. This study aimed to determine if PF by regulating EZH2/H3K27me3 modulated C-X-C chemokine receptor type 4…

The role of gut microbiota in live birth attainment upon in vitro fertilization (IVF) remains unclear. We recruited 67 women, evaluated bacterial constituents in the serum, and analyzed the gut microbiota composition and functions prior to …

Sheep (Ovis aries) are valuable biomedical models for studying human reproductive biology, however, the transcriptional dynamics underlying their ovarian development remain insufficiently characterized. In this study, we profiled 61,649 sin…

Although tumor-associated macrophages (TAMs) play a critical immunomodulatory role in colorectal cancer (CRC), the mechanisms underlying their polarization remain unclear. This study identifies the P2X4 receptor (P2X4R) as a crucial mediato…

The extracellular matrix (ECM) is a crucial component of the lung, providing both tissue stability and elasticity. While certain pathologic changes in the ECM, such as fibrotic foci, have long been recognized as hallmarks of pulmonary fibro…

In this pilot study, we investigated three minimally to noninvasive biospecimens: cervicovaginal lavages (CVLs), vaginal swabs, and urine, from 18 premenopausal women using untargeted metabolomics to inform future gynecologic research. Meta…

Non-invasive liquid biopsy is a promising strategy for ovarian cancer (OC) detection. We evaluated the feasibility and efficacy of a multi-analyte approach combining circulating tumor DNA (ctDNA) detection with protein biomarkers for early …

Conventional treatments for advanced endometriosis often have limited efficacy due to chemotherapy resistance, recurrence, and metastasis. This study analyzed clinical specimens to investigate the role of NXF1 in endometrial cancer (ECa) pr…

Colorectal cancer (CRC) is a common malignancy, and TRIM24, an E3 ubiquitin ligase, is a potential target for various cancers. This study found high TRIM24 expression in CRC, correlating with poor prognosis and disease progression. Oroxin A…

Although per- and polyfluoroalkyl substances (PFAS) exposure has been linked to endometriosis, this association remains controversial, and the underlying mechanisms are unclear. This study aimed to investigate this relationship and explore …

The endometrium, essential for reproduction, undergoes cyclical shedding, remodeling, and regeneration. Using a large endometrial transcriptomic dataset (n = 206), we identified RNA splicing and transcript isoform-level changes across the m…

The mechanisms underlying the pathophysiology of endometriosis, characterized by the presence of endometrium-like tissue outside the uterus, remain poorly understood. This study aimed to identify cell type-specific gene expression changes i…

Endometriosis affects a substantial number of women of reproductive age, yet current diagnostic methods rely on invasive procedures. To address this limitation, we investigated THBS1 as a potential biomarker and regulator of disease progres…

This study investigated the prognostic impact of lymph node dissection (LND) in patients with stage I ovarian endometrioid carcinoma (OEC) and aimed to identify the optimal LND range for improved survival. By analyzing data from the Surveil…

m6A regulators were demonstrated to modulate the functions of intestinal epithelial and immune cells in the ulcerative colitis. This study aimed to elucidate whether and how the m6A reader heterogeneous nuclear ribonucleoprotein C (HNRNPC) …

Uterine extracellular matrix (ECM) and uterine glands are essential parts of the endometrium, with important roles in remodeling associated with the estrous cycle, fertility, and pregnancy. Using second harmonic generation (SHG) microscopy,…

Endometriosis is a chronic multisystem disease associated with immunological, genetic, hormonal, psychological, and neuroscientific factors, leading to a significant socioeconomic impact worldwide. Though multidisciplinary management is the…

As advantageous as sexual reproduction is during progeny generation, it is also an expensive and treacherous reproductive strategy. The viviparous eukaryote has evolved to survive stress before, during, and after pregnancy. An important and…

Endometriosis is a benign condition characterized by the presence of ectopic endometrial tissue. Our study investigated the effect of endometriosis on the risk of endometrial cancer (EC) and the prognosis of endometriosis-associated EC. In …

Chronic pelvic pain (CPP) in women with no obvious pelvic pathology has few evidence-based treatment options. Our recent multicenter randomized controlled trial (GaPP2) in women with CPP and no obvious pelvic pathology showed that gabapenti…

Autographiviridae phage HH109 is a lytic Vibrio alginolyticus E110-specific phage, but the molecular mechanism underlying host recognition of this phage remains unknown. In this study, a transposon mutagenesis library of E110 was used to sh…

Somatic mutations contribute to cancer development by altering the activity of enhancers. In the study, a total of 135 mutation-driven enhancers, which displayed significant chromatin accessibility changes, were identified as candidate risk…

Existing medical treatments for endometriosis-related pain are often ineffective, underscoring the need for new therapeutic strategies. In this study, we applied a computational drug repurposing pipeline to stratified and unstratified disea…