Geraldine O. Canny (492710)

(A) In vivo magnetic resonance imaging (MRI), fat saturated T2-weighted Turbo spin echo images using a dedicated wrist coil at 3T: The red arrows indicate one experimentally induced-endometriotic implant in B6C3F1 and CD-1 nude mice. In a s…

(A) Four weeks after treatment, B6C3F1 (I, II) and CD-1 nude (III, IV) mice were sacrificed; endometrial implants were identified (black arrows) and excised for further analysis. Adhesions were also observed at implant sites in the B6C3F1 (…

Peritoneal endometriosis was surgically induced in mice divided into 3 groups (n = 7/group): Veh or LXA4 (5 μg/kg/mouse once daily) was administered from the day prior to surgery D(−1), or started at postoperative day 6 D(+6). Treatment was…

A. IL-1β, B. IL-6, C. FPR2, D. HIF-1α, E. COX-2, F. VEGF and G. MRP4 mRNA expression in endometriotic lesions was analyzed by qPCR. H. IL-1β, I. IL-6, J. FPR2, K. HIF-1α, L. COX-2, M. VEGF and N. MRP4 mRNA expression was similarly quantifie…

A. MMP-2, B. MMP-3, C. MMP-9, D. TGF-β1, E. TGF-β2, F. TGF-β3, G. MMP-2, H. MMP-9, I. TGF-β1, J. TGF-β2 gene expression in lesions and PFCs. Ten mice were used per group (Sham, Veh-, LXA4-treatment) and sacrificed 21 days after endometriosi…

mRNA was extracted from endometriotic lesions and PFCs and subjected to qPCR analysis to assess transcript levels of A. CYP19a1, B. ERα, C. GREB1, D. CCND1, E. c-Myc and F. ERα, G. CCND1, H. c-Myc, respectively. Results were normalized to G…

Negative and positive (breast cancer) controls were included to determine the specificity of the immunostainings (magnification: x100 and x200).

A. Representative pictures of a mouse with endometriotic lesions treated with vehicle (Veh, left panel) or LXA4 (right panel) for 21 days. Six uterine biopsies were implanted on the peritoneal wall, 3 on each side, in a minimum of 20 mice w…