Identification and analysis of induced endometriotic lesions.

Cássia G. T. Silveira (492705), Dominique Finas (492706), Peter Hunold (492707), Frank Köster (492708), Katharina Stroschein (492709), Geraldine O. Canny (492710), Gerhard Moldenhauer (135129), Peter Altevogt (223601), Achim Rody (190314), Daniela Hornung (332203)
2015 · doi:10.1371/journal.pone.0082512.g002 · W6885592699
other OA: green CC0
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Abstract

(A) Four weeks after treatment, B6C3F1 (I, II) and CD-1 nude (III, IV) mice were sacrificed; endometrial implants were identified (black arrows) and excised for further analysis. Adhesions were also observed at implant sites in the B6C3F1 (II) and CD-1 (IV) mice and involved the liver (L) and gut (G). (B) Hematoxylin-eosin staining of the endometriotic tissues derived from both mouse groups was performed for histological evaluation (magnification: x100). (C) The presence of L1CAM was observed in the endometriotic implants derived from autologous (I) and heterologous (II) models by immunohistochemistry. Negative (III) and positive (colon, squamous epithelium) (IV) controls are shown (magnification: x200).
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L1 Cell Adhesion Molecule as a Potential Therapeutic Target in Murine Models of Endometriosis Using a Monoclonal Antibody Approach Figure 2 Identification and analysis of induced endometriotic lesions. (A) Four weeks after treatment, B6C3F1 (I, II) and CD-1 nude (III, IV) mice were sacrificed; endometrial implants were identified (black arrows) and excised for further analysis. Adhesions were also observed at implant sites in the B6C3F1 (II) and CD-1 (IV) mice and involved the liver (L) and gut (G). (B) Hematoxylin-eosin staining of the endometriotic tissues derived from both mouse groups was performed for histological evaluation (magnification: x100). (C) The presence of L1CAM was observed in the endometriotic implants derived from autologous (I) and heterologous (II) models by immunohistochemistry. Negative (III) and positive (colon, squamous epithelium) (IV) controls are shown (magnification: x200).

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