The effect of broccoli extract and soy isoflavones on endometrial implants and oxidative parameters in female rat model of endometriosis

Soudabeh Sabetian, Parimah Archin Dialameh, Nader Tanideh, Behrouz Gharesi‐Fard, Moslem Ahmadi, Maryam Valibeigi, Perikala V. Kumar, Sarah Siahbani, Bahia Namavar Jahromi
In: Research Square · 2022 · doi:10.21203/rs.3.rs-2369250/v1 · W4311542063
preprint OA: green CC0 ⤵ 1 in-corpus citation
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⚙ AI-generated summary by claude@2026-06, 2026-06-09 ⓘ

Broccoli extract combined with soy isoflavones decreased the volume and histopathological grade of endometrial implants in a rat model of endometriosis, with broccoli extract alone improving superoxide dismutase levels.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-10 ⓘ

This preprint studied whether oral broccoli extract (BE) and/or soy isoflavones (SI) affect lesion growth and oxidative and inflammatory markers in a surgically induced rat model of endometriosis, comparing BE alone, SI alone, their combination, and diphereline. Forty mature female Sprague-Dawley rats were randomized to five 6-week treatment groups, after which endometrial implant volume and histopathology were assessed and serum oxidative parameters (SOD, MDA) and TNF-α were measured. The implants’ volume was significantly reduced in the diphereline group and histopathologic grades were significantly decreased in the BE/SI combination and diphereline groups versus control; SOD activity was increased in the BE group. Limitations include the preprint’s lack of journal peer review and the small group sizes (n=9), with incomplete detail in the provided text about all statistical outcomes (e.g., TNF-α and MDA results). This paper is centrally about endometriosis — it evaluates broccoli extract and soy isoflavones on endometrial implant growth, histology, and oxidative/inflammatory parameters in an endometriosis rat model.

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Abstract

Abstract Background: Endometriosis is a gynecological disorder characterized by the growth of endometrial tissue outside the uterine cavity that is associated with chronic pelvic pain, subfertility and 1-3% increased risk of ovarian cancer. The purpose of the study was to investigate the effect of broccoli extract (BE) alone and in combination with soy isoflavones (SI) on endometrial implants in female rat. Method: In this study, endometriosis was induced surgically in 40 mature female rats. The rats were divided into 5 groups that were treated by oral gavage for 6 weeks with 0.5 ml of saline 0.9%/day (control group), BE (3000 mg/kg/day), SI (50mg/kg/day), BE/soy isoflavones (BE 3000 mg/kg/day + soy isoflavones 50mg/kg/day) and diphereline as a standard medication (3 mg/kg) intramuscularly. At the end of treatments, the volume and histopathology of the endometrial implants were compared among the 5 groups. The serum levels of oxidative parameters including Superoxide dismutase (SOD), Malondialdehyde (MDA) and tumor necrosis factor alpha (TNF-α) were also compared between the groups. Results: The volume of the implants significantly decreased in diphereline group (p = 0.002). The histopathological grade of endometrial implants in BE/SI and diphereline group were significantly decreased compared to the control group (p = 0.001). The serum levels of SOD in BE group were enhanced significantly in comparison to the control group (p = 0.034). Conclusion: BE in combination with SI decreased the growth and histopathologic grades of transplanted endometrial implants. These herbal compounds may have the potential to be used as an alternative medication for the treatment of endometriosis

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endometriosischronic_pelvic_pain

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