Single-cell Transcriptomic Analysis of Eutopic Endometrium and Ectopic Lesions of Adenomyosis

Zhiyong Liu, Zhonghua Sun, Hongyun Liu, Weipin Niu, Na Liang, Xin Wang, Yanfei Wang, Yaxin Shi, Li Xu, Wei Shi
In: Research Square · 2020 · doi:10.21203/rs.3.rs-127243/v1 · W4243881766
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-07 ⓘ

Single-cell RNA sequencing of adenomyosis samples revealed increased cancer-, inflammation-, and motility-associated terms, suggesting epithelial-endothelial transition and vasculogenic mimicry formation contribute to the disease's progression.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This paper used single-cell RNA sequencing to compare control endometrium from hysteromyoma patients with eutopic endometrium and ectopic adenomyosis lesions from one adenomyosis patient, followed by validation in additional leiomyoma (n=3) and adenomyosis (n=3) samples using immunofluorescence and CD34-PAS double staining to assess vasculogenic mimicry. The authors report that ectopic lesions show increased epithelial–endothelial marker colocalization (EPCAM with PECAM1), presence of a cell subpopulation with high copy number variation and tumour-like features, and an epithelial-to-endothelial transition associated with PAS-positive/CD34-negative vasculogenic mimicry formations. They also identify VNN1- and EPCAM-positive epithelial subclusters with active cell motility, and describe enrichment of cancer-, cell motility- and inflammation-related processes, alongside proliferation and angiogenesis, in adenomyosis progression, while noting the limited scRNA-seq sampling (n=1 per key group). This paper is centrally about endometriosis and/or adenomyosis—adenomyosis—using single-cell profiling of eutopic endometrium versus ectopic adenomyosis lesions and validation of epithelial–endothelial transition and vasculogenic mimicry.

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adenomyosis

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