Overnight maturation of a metaphase I oocyte retrieved from a natural cycle using human tubular fluid: a case report

Chng MW, Michelle Wan Xing Chng, Matthew Sie Kuei Lau, Lau MS, Chan M, Melinda Chan, Heng Hao Tan, Tan HH, Nadarajah S, Sadhana Nadarajah
Journal of assisted reproduction and genetics · 2013 · doi:10.1007/s10815-012-9903-y · PMID:23239127 · PMC3553355
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⚙ AI-generated summary by claude@2026-07+body, 2026-07-09 ⓘ

This case report demonstrates that overnight in vitro maturation of a metaphase I oocyte retrieved from a natural cycle is achievable using human tubular fluid.

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This case report describes overnight in vitro maturation of a human oocyte retrieved from a natural cycle, using human tubular fluid as the culture medium, to mature a metaphase I oocyte. The authors report that maturation in this medium was achieved, supporting the feasibility of using human tubular fluid for oocyte maturation in an assisted reproduction context. As a single-patient report, the main limitation is that it does not establish effectiveness or generalizability across broader populations or outcomes. The paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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References Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1979;312(8085):366. Goldberg JM, Falcone T, Attaran M. In vitro fertilization update. Cleve Clin J Med. 2007;74(5):329–38. MacDougall MJ, Tan SL, Jacobs HS. In-vitro fertilization and the ovarian hyperstimulation syndrome. Hum Reprod. 1992;7(5):597–600. Van Voorhis BJ, Sparks AE, Allen BD, Stovall DW, Syrop CH, Chapler FK. Cost-effectiveness of infertility treatments: a cohort study. Fertil Steril. 1997;67(5):830–6. Pelinck MJ, Hoek A, Simons AH, Heineman MJ. Efficacy of natural cycle IVF: a review of the literature. Hum Reprod Update. 2002;8(2):129–39. Daya S, Gunby J, Hughes EG, Collins JA, Sagle MA, YoungLai EV. Natural cycles for in-vitro fertilization: cost-effectiveness analysis and factors influencing outcome. Hum Reprod. 1995;10(7):1719–24. Janssens RM, Lambalk CB, Schats R, Schoemaker J. Successful in-vitro fertilization in a natural cycle after four previously failed attempts in stimulated cycles: case report. Hum Reprod. 1999;14(10):2497–8. Janssens RM, Lambalk CB, Vermeiden JP, Schats R, Schoemaker J. In-vitro fertilization in a spontaneous cycle: easy, cheap and realistic. Hum Reprod. 2000;15(2):314–8. Moschini RM, Chuang L, Poleshchuk F, Slifkin RE, Copperman AB, Barritt J. Commercially available enhanced in vitro maturation medium does not improve maturation of germinal vesicle and metaphase I oocytes in standard in vitro fertilization cases. Fertil Steril. 2011;95(8):2645–7. Hardy K, Wright CS, Franks S, Winston RM. In vitro maturation of oocytes. Br Med Bull. 2000;56(3):588–602. Roberts R, Franks S, Hardy K. Culture environment modulates maturation and metabolism of human oocytes. Hum Reprod. 2002;17(11):2950–6. Sutton ML, Gilchrist RB, Thompson JG. Effects of in-vivo and in-vitro environments on the metabolism of the cumulus-oocyte complex and its influence on oocyte developmental capacity. Hum Reprod Update. 2003;9(1):35–48. de Araujo CH, Noqueira D, de Araujo MC, Martins Wde P, Ferriani RA, dos Reis RM. Supplemented tissue culture medium 199 is a better medium for in vitro maturation of oocytes from women with polycystic ovary syndrome women than human tubal fluid. Fertil Steril. 2009;91(2):509–13. Conflict of interest The authors declare that they have no conflict of interest. Author information Authors and Affiliations Corresponding author Additional information Capsule IVM could be achieved using human tubular fluid. Rights and permissions About this article Cite this article Chng, M.W.X., Lau, M.S.K., Chan, M. et al. Overnight maturation of a metaphase I oocyte retrieved from a natural cycle using human tubular fluid: a case report. J Assist Reprod Genet 30, 77–79 (2013). https://doi.org/10.1007/s10815-012-9903-y Received: Accepted: Published: Issue date: DOI: https://doi.org/10.1007/s10815-012-9903-y

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endometriosis

MeSH descriptors

In Vitro Oocyte Maturation Techniques Menstrual Cycle Metaphase Oocyte Retrieval Oocytes Adult Culture Media Embryo Transfer Endometriosis Endometriosis Female Humans Menstrual Cycle Oocyte Retrieval Oocytes Oocytes Pregnancy Pregnancy Outcome Sperm Injections, Intracytoplasmic

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