Non-invasive Diagnostic Innovations for Endometriosis Detection: A Comprehensive Approach Using Menstrual Blood

Peter Oppelt, Marlene Rezk, Clara Ganhör, Eva Scharnagl, Andreas Ian Lackner, Angelika Lackner
In: Geburtshilfe und Frauenheilkunde · 2025 · vol. 85 · doi:10.1055/s-0045-1812524 · W4416156068
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⚙ AI-generated summary by claude@2026-07+body, 2026-07-09 ⓘ

This study developed a method for stabilizing menstrual blood and identified ten RNA biomarkers from menstrual blood that can accurately diagnose endometriosis in a small validation set.

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⚙ AI-generated deep summary by claude@2026-07, 2026-07-09 · read from full text ⓘ

The paper studies non-invasive diagnostic approaches for endometriosis using menstrual blood, focusing on experimental optimization of RNA handling (purity and quantity) across menstrual cycle timing, collection time point, temperature, stabilizer volume, collection method, and RNA stability over time. It also analyzes RNA-sequencing data from endometrium and blood samples (n=1,142) to identify endometriosis-specific biomarkers, then validates a selected set using qPCR in an independent menstrual blood test set (n=20). The authors report that RNA purity/amount remained robust under varying laboratory conditions, including menstrual blood stabilization for up to 7 days across temperatures from −20 to +60°C, and that qPCR showed statistically significant biomarker differences between endometriosis and healthy controls. A stated caveat is the need for larger-scale validation beyond the current study sizes. This paper is centrally about endometriosis — it develops and validates menstrual-blood RNA biomarkers and sample-stabilization methods for non-invasive endometriosis detection.

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Subscribe to RSS DOI: 10.1055/s-0045-1812524 Non-invasive Diagnostic Innovations for Endometriosis Detection: A Comprehensive Approach Using Menstrual Blood Authors Topic: Allgemeine Gynäkologie Einleitung: Endometriosis affects approximately 10% of women globally and is often diagnosed after 5-10 years. A non-invasive diagnosis is urgently needed to reduce diagnostic delays and improve patient outcomes. Our study focuses on menstrual blood as medium for a non-invasive diagnosis Patient:innen und Methoden: We conducted experiments on RNA purity and the amount of RNA in menstrual blood by testing various factors, including the collection time point during the menstrual cycle, temperature, stabilizer volume, collection method, and RNA stability over time. We analyzed RNA-sequencing patient samples (n=1.142) from endometrium and blood and identified specific biomarkers for endometriosis. We validated our biomarkers with an independent test set of menstrual blood using qPCR. Ergebnisse: Our study gives insights on how to handle menstrual blood for laboratory purposes. We could confirmed RNA purity and amount across varying conditions, including collection time point, temperature, RNA extraction kit, and stabilizer volume. With our laboratory method we are able stabilize menstrual blood for up to 7 days at temperatures ranging from -20 to+60°C. We identified specific biomarkers for endometriosis. We ended up with a list of 10 biomarkers, excluding those without physiological relevance, and tested them in menstrual blood. The independent test set (n=20) confirmed statistically significant difference between endometrsiosis and healthy control using qPCR. These results highlight the robustness of our approach for non-invasive endometriosis diagnostics, acknowledging the need for larger-scale validation. Schlussfolgerung/Diskussion: The menstrual blood diagnostic kit represents a breakthrough in endometriosis diagnosis, offering women a pain-free, cost-effective, and accurate solution for early detection and treatment. This innovation could significantly reduce the diagnosis time from years to days. Publication History Article published online: 12 November 2025 © 2025. Thieme. All rights reserved. Georg Thieme Verlag KG Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany

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