{"paper_id":"f66bd4a0-1fc1-4e9f-95f9-8b808ead673e","body_text":"Subscribe to RSS\nDOI: 10.1055/s-0045-1812524\nNon-invasive Diagnostic Innovations for Endometriosis Detection: A Comprehensive Approach Using Menstrual Blood\nAuthors\nTopic: Allgemeine Gynäkologie\nEinleitung: Endometriosis affects approximately 10% of women globally and is often diagnosed after 5-10 years. A non-invasive diagnosis is urgently needed to reduce diagnostic delays and improve patient outcomes. Our study focuses on menstrual blood as medium for a non-invasive diagnosis\nPatient:innen und Methoden: We conducted experiments on RNA purity and the amount of RNA in menstrual blood by testing various factors, including the collection time point during the menstrual cycle, temperature, stabilizer volume, collection method, and RNA stability over time. We analyzed RNA-sequencing patient samples (n=1.142) from endometrium and blood and identified specific biomarkers for endometriosis. We validated our biomarkers with an independent test set of menstrual blood using qPCR.\nErgebnisse: Our study gives insights on how to handle menstrual blood for laboratory purposes. We could confirmed RNA purity and amount across varying conditions, including collection time point, temperature, RNA extraction kit, and stabilizer volume. With our laboratory method we are able stabilize menstrual blood for up to 7 days at temperatures ranging from -20 to+60°C. We identified specific biomarkers for endometriosis. We ended up with a list of 10 biomarkers, excluding those without physiological relevance, and tested them in menstrual blood. The independent test set (n=20) confirmed statistically significant difference between endometrsiosis and healthy control using qPCR. These results highlight the robustness of our approach for non-invasive endometriosis diagnostics, acknowledging the need for larger-scale validation.\nSchlussfolgerung/Diskussion: The menstrual blood diagnostic kit represents a breakthrough in endometriosis diagnosis, offering women a pain-free, cost-effective, and accurate solution for early detection and treatment. This innovation could significantly reduce the diagnosis time from years to days.\nPublication History\nArticle published online:\n12 November 2025\n© 2025. Thieme. All rights reserved.\nGeorg Thieme Verlag KG\nOswald-Hesse-Straße 50, 70469 Stuttgart, Germany","source_license":"CC0","license_restricted":false}