The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids

Fumiko Kawasaki, Yasushi Kawano, Zeshan Hasan, Hisashi Narahara, Isao Miyakawa
In: Clinical and Experimental Medicine · 2003 · vol. 3(1) , pp. 27–31 · doi:10.1007/s102380300012 · PMID:12748876 · W1971928811
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This study measured interleukin-6 and its soluble receptor in human follicular fluid, finding higher concentrations in mature oocytes and suggesting their role in ovulation and as maturation markers.

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This study measured interleukin-6 (IL-6) and IL-6 soluble receptor (sR) concentrations in human follicular fluid from 45 women undergoing in vitro fertilization and embryo transfer, and assessed associations with oocyte maturation. Using ELISA, the authors found IL-6 levels were significantly higher in follicular fluid than serum, while IL-6 sR levels were significantly lower; both IL-6 and IL-6 sR were higher in follicular fluid containing mature oocytes than immature oocytes. In cultured granulosa cells from follicular fluid, IL-6 production increased markedly after forskolin (24 h) and after longer treatment (48 h), suggesting cAMP-related regulation. The paper explicitly links its findings to preovulatory follicular growth and notes the potential marker value, but it does not state a causal role for IL-6 in ovulation beyond these associations. This paper does not explicitly discuss endometriosis or adenomyosis; it was included in the corpus via a keyword match in the upstream search index.

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Abstract

In order to investigate the role of interleukin-6 (IL-6) and interleukin-6 soluble receptor (sR) in human ovulation, we evaluated the concentrations in human follicular fluid and analyzed the correlation of IL-6 and IL-6 sR with oocyte maturation. The oocytes were obtained from the follicular fluid of 45 women undergoing in vitro fertilization and embryo transfer. The concentrations of IL-6 and IL-6 sR in follicular fluid were measured by ELISA. In addition, granulosa cells obtained from the follicular fluid were cultured and treated with forskolin and 12- o-tetradecanoylphorbol 13-acetate for 24-48 h. The concentration of IL-6 was significantly higher in the follicular fluid than in the serum (P<0.01). In contrast, the concentration of IL-6 sR was significantly lower in the follicular fluid than in the serum (P<0.001). The concentrations of IL-6 and IL-6 sR were significantly higher in the follicular fluid containing mature oocytes than in fluid containing immature oocytes (P<0.05). The production of IL-6 was markedly increased over the basal level after 24 h of treatment with forskolin (P<0.001) and 48 h of treatment (P<0.01) with cultured granulosa cells. Our data suggest that IL-6 and IL-6 sR may play an important role in follicular growth and development in human preovulatory processes. It is possible that IL-6 in particular may be regulated by cAMP. IL-6 and IL-6 sR might also be valuable biochemical markers in the evaluation of oocyte maturation.
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Abstract. In order to investigate the role of interleukin-6 (IL-6) and interleukin-6 soluble receptor (sR) in human ovulation, we evaluated the concentrations in human follicular fluid and analyzed the correlation of IL-6 and IL-6 sR with oocyte maturation. The oocytes were obtained from the follicular fluid of 45 women undergoing in vitro fertilization and embryo transfer. The concentrations of IL-6 and IL-6 sR in follicular fluid were measured by ELISA. In addition, granulosa cells obtained from the follicular fluid were cultured and treated with forskolin and 12-o-tetradecanoylphorbol 13-acetate for 24–48 h. The concentration of IL-6 was significantly higher in the follicular fluid than in the serum (P<0.01). In contrast, the concentration of IL-6 sR was significantly lower in the follicular fluid than in the serum (P<0.001). The concentrations of IL-6 and IL-6 sR were significantly higher in the follicular fluid containing mature oocytes than in fluid containing immature oocytes (P<0.05). The production of IL-6 was markedly increased over the basal level after 24 h of treatment with forskolin(P<0.001) and 48 h of treatment (P<0.01) with cultured granulosa cells. Our data suggest that IL-6 and IL-6 sR may play an important role in follicular growth and development in human preovulatory processes. It is possible that IL-6 in particular may be regulated by cAMP. IL-6 and IL-6 sR might also be valuable biochemical markers in the evaluation of oocyte maturation. Similar content being viewed by others Author information Authors and Affiliations Additional information Received: 6 July 2002 / Accepted: 18 December 2002 Correspondence to Y. Kawano Rights and permissions About this article Cite this article Kawasaki, F., Kawano, Y., Kosay Hasan, Z. et al. The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids. Clin Exp Med 3, 27–31 (2003). https://doi.org/10.1007/s102380300012 Issue date: DOI: https://doi.org/10.1007/s102380300012

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