{"paper_id":"eafee990-e587-4a13-8e95-766b172d80ad","body_text":"Abstract.\nIn order to investigate the role of interleukin-6 (IL-6) and interleukin-6 soluble receptor (sR) in human ovulation, we evaluated the concentrations in human follicular fluid and analyzed the correlation of IL-6 and IL-6 sR with oocyte maturation. The oocytes were obtained from the follicular fluid of 45 women undergoing in vitro fertilization and embryo transfer. The concentrations of IL-6 and IL-6 sR in follicular fluid were measured by ELISA. In addition, granulosa cells obtained from the follicular fluid were cultured and treated with forskolin and 12-o-tetradecanoylphorbol 13-acetate for 24–48 h. The concentration of IL-6 was significantly higher in the follicular fluid than in the serum (P<0.01). In contrast, the concentration of IL-6 sR was significantly lower in the follicular fluid than in the serum (P<0.001). The concentrations of IL-6 and IL-6 sR were significantly higher in the follicular fluid containing mature oocytes than in fluid containing immature oocytes (P<0.05). The production of IL-6 was markedly increased over the basal level after 24 h of treatment with forskolin(P<0.001) and 48 h of treatment (P<0.01) with cultured granulosa cells. Our data suggest that IL-6 and IL-6 sR may play an important role in follicular growth and development in human preovulatory processes. It is possible that IL-6 in particular may be regulated by cAMP. IL-6 and IL-6 sR might also be valuable biochemical markers in the evaluation of oocyte maturation.\nSimilar content being viewed by others\nAuthor information\nAuthors and Affiliations\nAdditional information\nReceived: 6 July 2002 / Accepted: 18 December 2002\nCorrespondence to Y. Kawano\nRights and permissions\nAbout this article\nCite this article\nKawasaki, F., Kawano, Y., Kosay Hasan, Z. et al. The clinical role of interleukin-6 and interleukin-6 soluble receptor in human follicular fluids. Clin Exp Med 3, 27–31 (2003). https://doi.org/10.1007/s102380300012\nIssue date:\nDOI: https://doi.org/10.1007/s102380300012","source_license":"CC0","license_restricted":false}