Androgen metabolism by human peritoneal macrophages

Coddington Cc, C C Coddington, Charles C. Coddington, Letterie GS, Gerald S. Letterie, G S Letterie, T A Klein, Thomas A. Klein, Klein TA, Winkel CA, Craig A. Winkel, C A Winkel
Steroids · 1988 · vol. 51(1-2) , pp. 143–161 · doi:10.1016/0039-128x(88)90190-0 · PMID:3242165 · W2019719997
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This paper investigates how human peritoneal macrophages metabolize androgens, revealing their capacity to produce androgens from precursor steroids.

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Abstract

Peritoneal macrophages (PM) were obtained by peritoneal dialysis from a regularly menstruating woman with renal failure. Macrophages (10(6) cells) were incubated at 37 degrees C for various periods of time (0-4 hr) in the presence of 14C-androstenedione or 3H-androstenedione and various concentrations (0.06-5.06 microM) of nonradiolabeled androstenedione (A). Testosterone (T) formed was purified by column chromatography, thin layer chromatography, acetylation, and recrystalization to constant 3H:14C ratios. The rate of formation of T from A was linear for nearly 2 hr. Conversion of A to T was linear at cell numbers in the incubation up to 1 x 10(6). The formation of T from A followed Michaelis-Menten kinetics at concentrations of A between 0.06 and 5.06 microM. The apparent Km of the enzyme for A was 0.75 microM and the Vmax for T formation from A in these cells was 33.9 pmol x hr-1 x 10(6) cells-1. PM were obtained also from normal patients (n = 6) and patients with endometriosis (n = 5). The rate of T synthesis from A in PM obtained from patients with endometriosis [527 +/- 263 pmol x hr-1 x 10(6) cells-1 (mean +/- SEM, n = 5)] was similar to that observed in PM obtained from normal patients [518 +/- 226 pmol x hr-1 x 10(6) cells-1 (mean +/- SEM, n = 6)]. We observed a near 30-fold variation in the rate of formation of T from A by PM obtained from different individuals (range 54 to 1580 pmol x hr-1 x 10(6) cells-1). Further study is needed to elucidate the physiologic significance of PM androgen metabolism and its relationship to reproductive function.

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Condition tags

endometriosis

MeSH descriptors

Androstenedione Macrophages Peritoneal Cavity Adult Androstenedione Cell Count Cells, Cultured Endometriosis Endometriosis Endometriosis Female Humans Macrophages Menstrual Cycle Peritoneal Cavity Peritoneal Dialysis Testosterone Testosterone Testosterone Time Factors

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