Conditioned medium from healthy women's endometrial stem cells improve inflammatory and stemness- expression genes in endometriosis women

Seyedeh Saeideh Sahraei, Ali Kowsari, Faezeh Davoodi Asl, Mohsen Sheykhhasan, Leila Naserpoor, Azar Sheikholeslami
In: Research Square · 2021 · doi:10.21203/rs.3.rs-249510/v1 · W4249926602
preprint OA: green CC0
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

Conditioned medium from healthy women's menstrual blood-derived stem cells reversed altered inflammatory and stemness gene expression in endometriosis-derived stem cells.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This preprint studied whether conditioned medium (CM) from menstrual blood-derived mesenchymal stem cells (MenSCs) of healthy women (NE-MenSCs) alters surface markers and the expression of inflammatory and stemness-related genes in MenSCs derived from women with endometriosis (E-MenSCs). E-MenSCs (n=3; stages III–IV) and NE-MenSCs (n=3; controls) were isolated from menstrual blood, cultured, and then exposed to NE-MenSC-derived CM; CD markers were assessed by flow cytometry and IL-1α, COX-2, HIF-1α, and OCT-4, NANOG, SOX2 were quantified by real-time PCR. Treatment shifted E-MenSC morphology toward that of normal cells and reduced CD10 expression, alongside significant changes in the inflammatory and stemness gene expression levels toward “normal” patterns. The main limitation explicitly stated is that the work is a Research Square preprint that has not been peer reviewed. This paper is centrally about endometriosis — it tests whether CM from healthy endometrial stem cells of menstrual origin can normalize inflammatory and stemness gene expression in endometriosis-derived MenSCs.

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Abstract

Abstract Background. Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium from Menstrual blood-derived stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Methods and Results. Endometriosis derived MenSCs (E-MenSCs) were treated with conditioned medium (CM) derived from healthy women’s MenSCs (NE-MenSCs). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. Results. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the IL-1, COX-2, and HIF-1\(\alpha\) as an inflamaturay genes and OCT-4, NANOG, and SOX2 as a stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Conclusions. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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endometriosis

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