Establishment and characterization of patient-derived endometriotic microtissues

L. S. Kesel, Hans Bösmüller, Christian Schmees, M. Weiss
In: Geburtshilfe und Frauenheilkunde · 2024 · vol. 84(10) , pp. e158 · doi:10.1055/s-0044-1790802 · W4403328446
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Patient-derived endometriotic microtissues (EPDM) were established and characterized, revealing specific signaling protein releases and lymphocyte populations, to serve as a relevant model for endometriosis research.

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Objective

In this study we aimed to establish and characterize patient-derived endometriotic microtissues (EPDM), capturing both epithelial layer characteristics and the microenvironment of endometriosis to closely mimic the in-vivo situation. In this way the EPDM can represent a suitable model for basic and applied research.

Material and methods

EPDM and endometriosis-infiltrating lymphocytes (EILs) were isolated from fresh tissue samples from endometriomas. Isolated EPDM were processed freshly as well as formalin-fixed paraffin-embedded specimen for subsequent immunohistochemistry, flow cytometry, proteome and secretome analysis.

Results

and summary: We established 4 EPDM models showing histopathological evidence of endometriotic origin. Live-dead staining and confocal microscopy confirmed high EPDM viability after the isolation procedure. Bead-based multiplex immunoassay analysis was applied to further characterize the signaling protein release of EPDM under basal conditions and after hormonal treatment. Numerous signaling proteins being released from our EPDM models were identified, including IL-6, IL-8, and YKL-40. DigiWest, a novel multiplexed protein profiling technology, provided further insights into endometriosis-associated protein signaling pathway activities. Flow cytometry analysis of the autologous EILs fraction determined the present lymphocyte panel of the endometriotic microenvironment.

Conclusion

EPDM represent a promising and clinically relevant model that can be used in the future to better understand endometriosis or to apply suitable therapies. Publication History Article published online: 01 October 2024 © 2024. Thieme. All rights reserved. Georg Thieme Verlag KG Rüdigerstraße 14, 70469 Stuttgart, Germany

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