{"paper_id":"d9ebbbfc-caf9-47af-acd1-7b8855bcdb6a","body_text":"Subscribe to RSS\nDOI: 10.1055/s-0044-1790802\nEstablishment and characterization of patient-derived endometriotic microtissues\nAuthors\nDespite intensive research, the development of endometriosis remains elusive. Highly painful symptoms, predominantly affecting young women, and the limited therapeutic options highlight the need to improve our understanding of disease biology and to advance early diagnostic and therapeutic strategies.\nObjective: In this study we aimed to establish and characterize patient-derived endometriotic microtissues (EPDM), capturing both epithelial layer characteristics and the microenvironment of endometriosis to closely mimic the in-vivo situation. In this way the EPDM can represent a suitable model for basic and applied research.\nMaterial and methods: EPDM and endometriosis-infiltrating lymphocytes (EILs) were isolated from fresh tissue samples from endometriomas. Isolated EPDM were processed freshly as well as formalin-fixed paraffin-embedded specimen for subsequent immunohistochemistry, flow cytometry, proteome and secretome analysis.\nResults and summary: We established 4 EPDM models showing histopathological evidence of endometriotic origin. Live-dead staining and confocal microscopy confirmed high EPDM viability after the isolation procedure. Bead-based multiplex immunoassay analysis was applied to further characterize the signaling protein release of EPDM under basal conditions and after hormonal treatment. Numerous signaling proteins being released from our EPDM models were identified, including IL-6, IL-8, and YKL-40. DigiWest, a novel multiplexed protein profiling technology, provided further insights into endometriosis-associated protein signaling pathway activities. Flow cytometry analysis of the autologous EILs fraction determined the present lymphocyte panel of the endometriotic microenvironment.\nConclusion: EPDM represent a promising and clinically relevant model that can be used in the future to better understand endometriosis or to apply suitable therapies.\nPublication History\nArticle published online:\n01 October 2024\n© 2024. Thieme. All rights reserved.\nGeorg Thieme Verlag KG\nRüdigerstraße 14, 70469 Stuttgart, Germany","source_license":"CC0","license_restricted":false}