Soluble factors formed during the healing of the endometrium suppress its "fibrosis” in vitro

Roman Eremichev, Olga Grigorieva, Konstantin Kulebyakin, Anastasia Efimenko, П. И. Макаревич
In: Genes & Cells · 2018 · vol. 13(2) , pp. 63–66 · doi:10.23868/201808021 · W2894465023
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Abstract

During each period, the uterine mucosa of women of reproductive age heals without fibrosis. Previously, we established that the soluble factors that are released in this way have an antifibrotic effect on the culture of the human endometrial mesenchymal stromal cells. The objective of this work was to evaluate the antifibrotic properties of these factors on the in vitro endometrial fibrosis model. Serum menstrual and peripheral blood were obtained from a healthy donor in one day. Mesenchymal stromal cells of the endometrium were also isolated from menstrual blood. Simulation of endometrial fibrosis in vitro was carried out by differentiation of endometrial mesenchymal stromal cells into myofibroblasts under the action of TGF-ß1 (5 ng/ml). Evaluation of the effectiveness of the menstrual blood serum antifibrotic effect on the endometrial mesenchymal stromal cells and myofibroblasts derived from them was carried out by analyzing the expression of а-smooth muscle actin by immunofluorescence. Serum of peripheral blood with equal protein concentration was used as a control. Menstrual blood serum reduces the number of stress-fibrils positive for а-smooth muscle actin (a marker of myofibroblasts), both in the culture of endometrial mesenchymal stromal cells, and in in vitro modeling of endometrial fibrosis using TGF-ß1. These results indicate the presence of soluble factors in the serum of menstrual blood with antifibrotic properties. Perhaps their identification will explain the mechanisms of endometrial healing not accompanied by fibrosis. In addition, it can help to identify the causes of fibrosis of the uterine lining in gynecological diseases and develop effective methods for their treatment.

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