Additional file 2: of Nodal induces apoptosis and inhibits proliferation in ovarian endometriosis-clear cell carcinoma lesions

Figure S2. Overexpression of Nodal enhances susceptibility to apoptosis in OEmCa cells. (A) After treatment of the stable Nodal-expressing Ish-NoD and mock cells with 1 μg/mL Dox for the times indicated, cells undergoing apoptosis (sub-G1) were detected by flow cytometry. This experiment was performed in triplicate using independent samples. (B) Upper: after treatment with 1 μg/mL Dox, the stable Nodal-expressing Ish-NoD and mock cells undergoing apoptosis (indicated by arrows) were detected by TUNEL assay. Original magnification, × 400. Lower: number of apoptotic cells per 10 high power fields (HPFs) detected by TUNEL assay for the times shown. (C) Left: western blot analysis of the indicated proteins in the stable Nodal-expressing Ish-NoD and mock cells after 1 μg/mL Dox treatment for the times shown. Right: values of endogenous Bcl2 relative to Bax protein were calculated by normalization to β-actin in the stable Nodal-expressing Ish-NoD and mock cells after 1 μg/mL Dox treatment for the times shown. (TIF 3448 kb)