Effect of Genistein on Proliferating Cell Nuclear Antigen (PCNA) Expression and Vascular Density in The Peritoneum of Endometriosis Mice Model

Anggia Prameswari Wardhana, Yahya Irwanto
In: Asian Journal of Health Research · 2023 · vol. 2(3) , pp. 5–11 · doi:10.55561/ajhr.v2i3.104 · W4389921845
article OA: diamond CC0 ⤵ 3 in-corpus citations
🔓 Open OA copy Full text JSON View on OpenAlex View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-07 ⓘ

Genistein administration in an endometriosis mouse model significantly reduced vascular density and modulated proliferating cell nuclear antigen expression in a dose-dependent manner.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 · read from full text ⓘ

This experimental study evaluated the effects of genistein at four doses (1.3, 1.95, 2.6, and 3.25 mg/day) on proliferating cell nuclear antigen (PCNA) expression and vascular density in mouse peritoneal tissue using immunohistochemical assessment and hematoxylin-eosin for vascularity, with ANOVA and Dunnett T3 used for analysis. In endometriosis mice, genistein produced significant changes in both PCNA expression and vascular densities (p=0.001, p<0.05), with PCNA increasing at doses of 1.3, 1.95, and 2.6 mg/day and showing an apparent reduction at 3.25 mg/day while vascular densities were significantly reduced in all treatment groups. A stated caveat is that the work used stored biological materials and details of lesion characterization beyond peritoneal tissue assessment are limited. This paper is centrally about endometriosis — it tests genistein’s impact on PCNA (proliferation) and vascular density in an endometriosis mouse model.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Introduction: Survival of endometriosis lesions depends on the formation of blood vessels, cell proliferation, and oxygen supply. Their vascular densities can evaluate the vascularity of ectopic endometriosis tissues. Proliferating Cell Nuclear Antigen (PCNA) is one of the markers of cell proliferation that affects the growth of endometriotic lesions. Genistein is an isoflavone component that highly potential effect as an anti-angiogenic and inhibiting proliferation through the binding capacity to estrogen receptors. Material and Methods: This experimental study uses the Stored Biological Materials of mice peritoneum tissue. The paraffin block was divided into six groups, which are the negative control group, positive control group, and four treatment groups with genistein doses of 1.3 mg/day, 1.95 mg/day, 2.6 mg/day, and 3.25 mg/day. Immunohistochemical techniques and hematoxylin-eosin for vascular densities examined PCNA expressions. Preparation analysis uses OLIVIA software. Preparation calculations are done manually with cell count software. Observations were analyzed with the ANOVA test and Dunnett T3 5% test. Results: Based on analysis with ANOVA, there was a significant difference in PCNA expression and vascular densities because of the effect of genistein in various doses with a p-value of 0.001 (p<0.05). Based on Dunnett T3 5% test increase of PCNA expression by giving genistein doses 1,3 mg/day, 1,95 mg/day, and 2,6 mg/day, a reduction in dose of 3,25 mg/day. Vascular densities reduced significantly in all treatment groups. Conclusion: Administration of genistein in various doses significantly increases PCNA expression but reduces PCNA expression in a dose of 3.25 mg/day and significantly reduces vascular densities.
Full text 2,154 characters · extracted from oa-doi-fallback · 5 sections · click to expand

Keywords

Endometriosis, Genistein, PCNA, Vascular densityAbstract

Introduction

Survival of endometriosis lesions depends on the formation of blood vessels, cell proliferation, and oxygen supply. Their vascular densities can evaluate the vascularity of ectopic endometriosis tissues. Proliferating Cell Nuclear Antigen (PCNA) is one of the markers of cell proliferation that affects the growth of endometriotic lesions. Genistein is an isoflavone component that highly potential effect as an anti-angiogenic and inhibiting proliferation through the binding capacity to estrogen receptors.

Material and methods

This experimental study uses the Stored Biological Materials of mice peritoneum tissue. The paraffin block was divided into six groups, which are the negative control group, positive control group, and four treatment groups with genistein doses of 1.3 mg/day, 1.95 mg/day, 2.6 mg/day, and 3.25 mg/day. Immunohistochemical techniques and hematoxylin-eosin for vascular densities examined PCNA expressions. Preparation analysis uses OLIVIA software. Preparation calculations are done manually with cell count software. Observations were analyzed with the ANOVA test and Dunnett T3 5% test.

Results

Based on analysis with ANOVA, there was a significant difference in PCNA expression and vascular densities because of the effect of genistein in various doses with a p-value of 0.001 (p<0.05). Based on Dunnett T3 5% test increase of PCNA expression by giving genistein doses 1,3 mg/day, 1,95 mg/day, and 2,6 mg/day, a reduction in dose of 3,25 mg/day. Vascular densities reduced significantly in all treatment groups.

Conclusion

Administration of genistein in various doses significantly increases PCNA expression but reduces PCNA expression in a dose of 3.25 mg/day and significantly reduces vascular densities. Downloads Published Issue Section License Copyright (c) 2023 Anggia Prameswari Wardhana, Yahya Irwanto This work is licensed under a Creative Commons Attribution 4.0 International License.

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

endometriosis

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (14)

Cited by (3)

Source provenance

openalex
last seen: 2026-06-10T17:14:06.276822+00:00
License: CC0 · commercial use OK