The role of mir-214-5p and mir-548-5p expressions in endometriosis

Fariba Dehghani Ashkezari, Seyed Hamidreza Mirabutalebi, Emad Babakhanzadeh, Nasrin Ghasemi
Molecular biology reports · 2024 · vol. 51(1) , pp. 1148 · doi:10.1007/s11033-024-10066-x · PMID:39535657 · W4404326204
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-07 ⓘ

This study found decreased miR-214-5p and increased miR-548-5p expression in endometriosis tissues compared to normal controls.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study examined expression changes of the microRNAs miR-214-5p and miR-548-5p by comparing ectopic and eutopic endometrial tissues with normal endometrium in 45 women (15 eutopic, 15 ectopic, 15 healthy controls) from Shahid Sadoughi Hospital, using RNA extraction followed by two-step qRT-PCR and fold-change comparison with two-way ANOVA. The authors found significant down-regulation of miR-214-5p and significant up-regulation of miR-548-5p in endometriosis samples relative to controls. The paper concludes that these miRNAs may be involved in endometriosis pathogenesis, while also noting that no datasets were generated or analyzed during the study. This paper is centrally about endometriosis — specifically measuring differential expression of miR-214-5p and miR-548-5p in ectopic versus eutopic versus normal endometrial tissues.

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Abstract

BACKGROUND: Endometriosis is a benign gynecological disease that affects about 1% of all women and up to 15% of women of childbearing age. To date, none of the proposed theories exhaustively explain the pathophysiology of the disease or the associated clinical manifestations. As part of efforts to introduce new methods for the early and non-invasive diagnosis of endometriosis, this project investigated changes in the expression of miR-214-5p and miR-548-5p in ectopic and eutopic tissue compared to normal endometrial tissue. MATERIALS AND METHODS: Forty-five samples (15 eutopic, 15 ectopic and 15 healthy controls) from women referred to Shahid Sadoughi Hospital (Yazd, Iran). RNA extraction was performed using an RNA extraction kit, and cDNA was synthesized. Two-step qRT-PCR was performed according to the manufacturer's instructions. GraphPad Prism 8 software and Two-Way ANOVA test were used to compare fold-change expression. RESULTS: The results indicate a significant down-regulation of miR-214-5p expression levels (P-value < 0.05) and an increase in miR-548-5p expression levels (P-value < 0.05) in endometriosis samples compared to those in control tissues. CONCLUSION: miR-214-5p and miR-548-5p may regulate the pathogenesis of endometriosis. The down-regulation of miR-214-5p in people with endometriosis compared to healthy individuals may indicate its suppressive role. The upregulation of miR-548-5p could confirm the oncogenic role of this microRNA in endometriosis. The development of new therapeutic strategies targeting these miRNAs could be promising in the treatment of this disease.

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Condition tags

endometriosis

MeSH descriptors

Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis Endometriosis MicroRNAs MicroRNAs MicroRNAs

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