{"paper_id":"c52d58f2-802f-4f96-89f8-df9514bfb419","body_text":"Abstract\nBackground\nEndometriosis is a benign gynecological disease that affects about 1% of all women and up to 15% of women of childbearing age. To date, none of the proposed theories exhaustively explain the pathophysiology of the disease or the associated clinical manifestations. As part of efforts to introduce new methods for the early and non-invasive diagnosis of endometriosis, this project investigated changes in the expression of miR-214-5p and miR-548-5p in ectopic and eutopic tissue compared to normal endometrial tissue.\nMaterials and methods\nForty-five samples (15 eutopic, 15 ectopic and 15 healthy controls) from women referred to Shahid Sadoughi Hospital (Yazd, Iran). RNA extraction was performed using an RNA extraction kit, and cDNA was synthesized. Two-step qRT-PCR was performed according to the manufacturer’s instructions. GraphPad Prism 8 software and Two-Way ANOVA test were used to compare fold-change expression.\nResults\nThe results indicate a significant down-regulation of miR-214-5p expression levels (P-value < 0.05) and an increase in miR-548-5p expression levels (P-value < 0.05) in endometriosis samples compared to those in control tissues.\nConclusion\nmiR-214-5p and miR-548-5p may regulate the pathogenesis of endometriosis. The down-regulation of miR-214-5p in people with endometriosis compared to healthy individuals may indicate its suppressive role. The upregulation of miR-548-5p could confirm the oncogenic role of this microRNA in endometriosis. The development of new therapeutic strategies targeting these miRNAs could be promising in the treatment of this disease.\nSimilar content being viewed by others\nData availability\nNo datasets were generated or analysed during the current study.\nReferences\nMoein Mahini S, Younesi M, Mortazavi G, Samare-Najaf M, Karim Azadbakht M, Jamali N. 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International Journal of Molecular Sciences. 2023;24(24):17470.\nAcknowledgements\nWe would like to thank our research colleagues from the Yazd Institute of Reproductive Sciences for their assistance to this project.\nFunding\nNot applicable.\nAuthor information\nAuthors and Affiliations\nContributions\nF.D. performing the main steps of essay, writing the manuscript and statistical tests. E.B. and H.M. Collecting the samples and helping to perform RNA extraction and qPCR. N.G. Head of team and monitoring and fixing technical errors during all steps of the study. The authors read and approved the final manuscript.\nCorresponding author\nEthics declarations\nEthics approval and consent to participate\nThis study approved by the Ethics Committee of the Shahid Sadoughi University of Medical Sciences (ethics code: IR.SSU.MEDICINE.REC1401.215), Yazd and written informed consent was obtained from each participant before the collection of tissue samples.\nConsent for publication\nPatients signed informed consent regarding publishing their data.\nCompeting interests\nThe authors declare no competing interests.\nAdditional information\nPublisher’s note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nSpringer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.\nAbout this article\nCite this article\nAshkezari, F.D., Mirabutalebi, S.H., Babakhanzadeh, E. et al. The role of mir-214-5p and mir-548-5p expressions in endometriosis. Mol Biol Rep 51, 1148 (2024). https://doi.org/10.1007/s11033-024-10066-x\nReceived:\nAccepted:\nPublished:\nVersion of record:\nDOI: https://doi.org/10.1007/s11033-024-10066-x","source_license":"CC0","license_restricted":false}