N6-methyladenosine methylation regulators can serve as potential biomarkers for endometriosis related infertility

Yalun He, Jie Ding, Tonglin Bai, Yangshuo Li, Xiaolan Liang, X Liang, Yiming Chen, Yi‐Jiun Lin, Yi Lin, Wen Cheng, Chaoqin Yu
Biomolecules & biomedicine · 2024 · vol. 25(7) , pp. 1540–1552 · doi:10.17305/bb.2024.11311 · PMID:39739380 · PMC12097398 · W4405774396
article OA: gold CC0
📄 Open PDF View on OpenAlex View on PubMed View at publisher
⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

This study identified seven N6-methyladenosine regulators with diagnostic value for endometriosis-related infertility, revealing distinct molecular clusters associated with immune pathways and identifying HNRNPA2B1 and HNRNPC as potential biomarkers.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study investigated how m6A methylation regulators relate to endometriosis-associated infertility by analyzing public transcriptomic datasets (GSE120103 for regulator identification and ROC diagnostics, plus cluster-based differential expression with GO/KEGG and immune infiltration analyses). It identified seven key m6A regulators with diagnostic value for EMS-related infertility and defined two distinct m6A molecular clusters whose differentially expressed genes were enriched for immune pathways, with regulator expression linked to differences in immune cell infiltration. Single-cell analysis of GSE214411 showed elevated HNRNPA2B1 and HNRNPC in endometrial immune cells from infertile EMS patients, with decreased levels in stromal cells, and immunohistochemistry in clinical samples found higher HNRNPA2B1 and HNRNPC in eutopic endometrium of fertile women than in ovarian EMS patients. This paper is centrally about endometriosis—specifically identifying m6A regulators (HNRNPA2B1, HNRNPC and others) as potential biomarkers for endometriosis-related infertility.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Endometriosis (EMS) is a chronic inflammatory disease frequently associated with infertility. N6-methyladenosine (m6A) methylation, the most common form of methylation in eukaryotic mRNAs, has gained attention in the study of female reproductive diseases, including EMS and infertility. This study aimed to investigate the role of m6A regulators in EMS-related infertility. To begin, specific m6A regulators were identified by analyzing the GSE120103 dataset, followed by receiver operating characteristic (ROC) curve analysis. A nomogram model was then constructed, and unsupervised clustering of m6A regulators was performed to identify distinct m6A molecular clusters. Functional enrichment analysis of differentially expressed genes (DEGs) between these clusters, along with immune cell infiltration analysis, was subsequently conducted. In addition, the single-cell dataset GSE214411 was analyzed to explore the role of m6A regulators in various cell types. Finally, clinical samples were collected, and immunohistochemistry analysis was performed. The study identified seven key m6A regulators with significant diagnostic value for EMS-related infertility and two distinct m6A molecular clusters. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs between the clusters revealed that m6A clustering was strongly associated with immune pathways. Immune cell infiltration analysis further demonstrated that the expression levels of m6A regulators had a notable impact on immune cell infiltration. Single-cell analysis revealed that HNRNPA2B1 and HNRNPC were significantly elevated in endometrial immune cells from infertile EMS patients but notably decreased in stromal cells. Immunohistochemical staining confirmed that HNRNPA2B1 and HNRNPC expression levels were significantly higher in the eutopic endometrium of fertile women compared to ovarian EMS patients. These findings suggest that m6A regulators play critical roles in the development and progression of EMS-related infertility. Notably, HNRNPA2B1 and HNRNPC may serve as potential biomarkers for this condition.

My notes (saved in your browser only)

Condition tags

endometriosisinfertility

MeSH descriptors

Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine Adenosine

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (21)

Source provenance

europepmc
last seen: 2026-06-15T06:13:43.845377+00:00
openalex
last seen: 2026-06-10T17:14:06.276822+00:00
pmc
last seen: 2026-05-13T20:22:03.195721+00:00
pubmed
last seen: 2026-06-15T06:11:05.718204+00:00
License: CC0 · commercial use OK