Silencing of SEPT9 inhibits proliferation and migration of endometriotic stromal cells via suppression of the PI3K/AKT signaling pathway

Qiong Shi, Shi Q, Zi-Yi Xiong, Xiong ZY, Wang YH, Yuan-Hui Wang, Xu SY, Shi-Yao Xu, Li Wang, Wang L, Luo JM, Jia-Meng Luo, Li KX, Kong-Xian Li, Chen XY, Xiao-Yan Chen, Zhang S, Shun Zhang, Qi Chen, Chen Q, Jun-Hui Wan, Wan JH, Junhui Wan
Revista de investigacion clinica; organo del Hospital de Enfermedades de la Nutricion · 2026 · vol. 78(5) , pp. 100052 · doi:10.1016/j.ric.2026.100052 · PMID:42607562 · W7203649073
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Silencing SEPT9 inhibits proliferation and migration while promoting apoptosis in endometriotic stromal cells by suppressing the PI3K/AKT signaling pathway, which is upregulated in endometriosis tissues.

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Abstract

OBJECTIVE: The aim of this study was to investigate the effects of SEPT9 on proliferation, migration, and apoptosis in endometriotic stromal cells and to explore the underlying mechanisms. METHODS: Immunohistochemistry and western blotting revealed that SEPT9 expression was significantly elevated in ectopic and eutopic endometrial tissues from patients with endometriosis compared with normal endometrial tissues (p<0.05). Using immortalized human eutopic and ectopic endometrial stromal cells, genetic recombination was applied to overexpress SEPT9 in eutopic cells and knock down SEPT9 in ectopic cells. RESULTS: Transwell migration, EdU proliferation, and flow cytometry assays showed that SEPT9 overexpression enhanced cell proliferation and migration, whereas SEPT9 silencing reduced these behaviors and increased apoptosis. Pathway analyses identified the PI3K/AKT signaling pathway as a downstream target of SEPT9. Consistently, western blotting and qRT-PCR following SEPT9 knockdown confirmed decreased expression of PI3K/AKT-related proteins and genes. CONCLUSION: In conclusion, silencing SEPT9 inhibited proliferation and migration and promoted apoptosis in endometriotic stromal cells, potentially through suppression of the PI3K/AKT signaling pathway.

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