Endometriosis and ovarian cancer: Their association and relationship

E B-E participated in the surgery and follow-up of patients, performed the review, constructed tables and figures, and wrote the manuscript. M P-A participated in the revision of the cases, helped with the bibliographic search, and reviewed the manuscript. GA G-M reported the results of the histopathological and immunohistochemical studies and reviewed the manuscript. MI A participated in the surgery and follow-up of patients from San Juan University Hospital and reviewed the manuscript. P A performed the surgery and follow-up of patients from San Juan University Hospital, designed the study, constructed tables and figures and reviewed the manuscript. E B-E and P A had full access to all study data and take responsibility for the integrity of the data and the accuracy of the data analysis.

Our results show that the patients with OET and associated endometriosis represent 10.6% of cases with borderline or invasive OETs but they are younger, with lower parity, and more frequently premenopausal than those in patients with OET without associated endometriosis. In addition, the first showed a lower elevation of the CA-125 and CA-19-9 tumor markers and more frequently were borderline tumors, but the differences were not statistically significant. There were statistically significant differences in the lower tumor stage of the invasive cases, which in the majority of the OETs with associated endometriosis were type I of the Kurman and Shih's classification [ 24 , 25 ]. They also had higher possibility of optimal cytoreduction, and generally, therefore, they have a better prognosis and greater chances of survival, although they may also more frequently have an associated endometrial carcinoma. Histologically, in the majority of patients with OET and associated endometriosis, the ovarian tumor is of endometrioid (53%) or clear cell (22%) lineage; and in most cases with associated endometriosis with serous or mucinous OET, it was more frequently borderline, which also justifies the better prognosis of OETs associated with endometriosis. From the immunohistochemical point of view, estrogen-receptors are generally positive in associated endometriosis and in endometrioid tumors and negative in the clear cell tumor component of these OETs with associated endometriosis. The determination of p53 protein was generally positive in the clear cell tumor and in associated endometriosis, possibly atypical. Furthermore, two cases with previous histologically confirmed endometriosis were followed-up to assess their evolution to borderline endometrioid carcinoma (after frequent episodes of recurrence) and clear cell carcinoma (after acute inflammatory episode), respectively, showing persisting foci of endometriosis, good postoperative evolution, and being also the clinical and immunohistochemical data concordant with the previously exposed. All these findings could suggest that in certain women with benign endometriosis, inflammatory or other intercurrent factors could induce a variable malignant transformation towards endometrioid or clear cell tumor, depending on such factors and their action, among others, about the hormonal receptors, as indicated by several authors [ [26] , [27] , [28] , [29] , [30] , [31] , [32] ]. The main limitation was the retrospective analysis of cases presenting OET and associated endometriosis operated at two different hospitals over a long period of time (1995–2015), in which the criteria for pathologists to categorize borderline tumors and cases with atypical endometriosis may have differed according to hospital and over time. However, a significant number of cases showing OET and endometriosis were analyzed (n = 36); and also there were statistically significant differences with the cases without associated endometriosis (n = 305), similar to the results of other studies [ 7 , 33 , 34 ]. In addition, we also analyzed hormonal receptors and p53 protein expression in endometriosis and endometrioid or clear cell tumors in a small number of cases and described the evolution of two interesting cases with previous histologically confirmed endometriosis that later evolved into a borderline endometrioid tumor and clear cell carcinoma, respectively. Since Sampson [ 14 ], many studies have analyzed the relationship between endometriosis and OETs, with a reported frequency of this association of 8–12% [ 19 , 20 , 35 ], with a higher incidence in cases of endometrioid and clear cell OET. In addition, endometriosis is considered a risk factor for ovarian carcinoma (RR 4.6; 95% CI, 1.12–12.11) [ 36 ], although their association has also been considered a good prognostic factor. Some authors [ 11 , 13 , 20 , 37 ] have reported more favorable characteristics in endometriosis-associated OETs, such as a higher proportion of low-grade and Kurman and Shih’s type I tumors, less elevated serologic marker values, and higher survival rates; however, the presence of synchronous endometrial cancer besides OET + endometriosis has been also reported [ 38 ]. We also observed this in our data, in which 33.3% of endometriosis-associated OETs with the endometrioid subtype also presented synchronous endometrioid endometrial cancer. Inflammation and the effect of sex steroids are two factors that may play a role in the development of clear cell and endometrioid OET [ 3 , 26 , 27 , 39 , 40 ]. Estrogens appear to be a mitogen for endometriosis and estrogen-receptor positivity is observed in endometrioid OET [ 3 , 41 ]. In addition, a link between inflammation and estrogen production in endometriosis has been observed, describing a positive feedback cycle that favors overexpression of key steroidogenic genes, most notably aromatase, overexpression of COX2, and continuous local production of estradiol and PGE2 in endometriotic tissue [ [42] , [43] , [44] , [45] ]. Moreover, most endometrioid carcinomas present functioning stroma and in situ production of estrogens [ 3 , 39 , 46 , 47 ]. However, clear cell OETs do not present estrogen-receptor positive expression in the tumor tissue [ 29 ]; thus, endometriosis could develop into clear cell (hormone-independent) OET if the hormone receptors are destroyed during malignant transformation [ 21 , 48 ]. Various authors [ 27 , 28 , 30 , 32 ] have suggested a dualistic model of progression and development from endometriosis to OET, as follows: 1) endometriosis-associated endometrioid (estrogen- and progesterone-receptor positive), and 2) endometriosis-associated clear cell (estrogen-receptor negative and progesterone-receptor negative, perhaps associated with iron-mediated oxidative stress) [ 31 ]. It has also been suggested that the chronic inflammation caused by endometriosis [ 27 ], together with genetic changes (mutation of the p53 tumor suppressor gene), may promote progression to an endometriosis-associated OET and p53 mutations are commonly found in endometriosis-associated endometrioid (21–42%) and clear cell (0–27.3%) OETs [ 39 , 49 ]. The results our immunohistochemical studies indicate the same, suggesting that inflammatory processes may destroy the hormone receptors, favoring progression to clear cell OETs. However, in the endometrioid subtype, estrogen-receptor, progesterone-receptor, and p53 expression remain positive in both endometriotic and tumor tissues, suggesting local hormone production in the functioning tumor stroma or stromal hyperplasia in the healthy residual ovary, which could favor malignant transformation. The findings of the two patients with a diagnosis and histological confirmation of endometriosis prior to the development of endometriosis-associated OET also suggest a greater risk of developing ovarian endometrioid (hormone-dependent) carcinoma in cases of endometriosis with frequent episodes of recurrence and a greater increase in CA-19-9. However, Case 2 presented acute inflammatory episodes and hemorrhagic ascites (possibly associated with iron-mediated oxidative stress), with a severely increased blood sedimentation rate, which could promote the downregulation of estrogen-receptors and multifocal transformation of endometriosis into a hormone-independent clear cell carcinoma. The eventual outcome could be a complete transformation into either endometrioid or clear cell carcinoma (type I tumors). Indeed, Kurman and Shih [ 25 ] have pointed out that “type I tumors develop from benign extraovarian lesions that implant on the ovary and which can undergo malignant transformation, whereas many type II carcinomas develop from intraepithelial carcinomas in the fallopian tube and, as a result, disseminate as carcinomas that involve the ovary and extraovarian sites, which probably accounts for their clinically aggressive behavior”. In summary , our findings and review of the literature suggest that patients with surgical findings of OET and associated endometriosis could correspond to an intermediate stage within the possible progression and malignant transformation of endometriosis to endometrioid or clear cell carcinomas or other type I carcinomas without associated endometriosis.

The characteristics of two subseries of studied patients with OETs (131 cases from Hospital Marina Baixa and 210 from San Juan University Hospital) are shown in Table 1 . There were no significant differences in the data corresponding to age, parity, menopause status, tumor markers, invasiveness, histological subtype, atypical endometriosis, associated endometrial carcinoma, and survival between the subgroups. However, it was significantly more frequent stage III, and less frequent stage I, in series 2. Subsequently, the survival at 5 years in series 2 was somewhat lower, but without reaching statistical significance. Table 1 Baseline characteristics of studied patients with ovarian epithelial tumors. Table 1 Characteristics Series 1: Patients operated in HMB, 131 cases Series 2: Patients operated in SJUH, 210 cases Age, years, m ± SD (range) 55,9±14,8 (45-87) 56,2±14,6 (44-88) Parity: Nulliparous, n (%) 37 (28,2) 62 (29,5) ≥ Primiparous, n (%) 94 (71,8) 148 (70,5) Menopause age, (n) m ± SD (range) (79) 48,7±4,6 (36-57) (90) 49,1±3,8(35-57) Premenopausal, (n, %) 45 (34,4) 78 (37,1) Postmenopausal, (n, %) 86 (65,6) 132 (62,9) CA-125 U/mL (n), m ± SD (114) 521,8±1085,4 (107) 627,5±1383,8 CA-19−9 U/mL (n), m ± SD (109) 638,3±4873,1 (104) 332,0±1194,7 BSR mm/h (n), m ± SD (77) 35,9±32,4 (103) 35,9±26,2 Borderline n (%) 36 (27,5) 67 (31,9) Invasive (n, %) 95 (72,5) 143 (68,1) With associated 15 cases (11.5%) 21 cases (10%) endometriosis, n(%) [4 (26.7)/11 (73.3)] [9 (42.8)/12 (57.1)] [BL/Inv] Atypical endometriosis or p53+ p53+ = 3(20) Atypical end = 5(23.8) Associated endometrial Ca in End/No end 3/15(20%)/7/116(6%) 3/21(14.3%)/8/189(4,2%) Type I tumors, n (%)/[Inv] 84 (64,1)/[48] 116 (55,2)/[50] Type II tumors, n (%)/[Inv] 47 (35,9)/[47] 94 (44,8)/[93] Endometrioid, CC, and mix/[Inv[] 35 (26,7)/[34] 39 (18,6)/[33] Serous, mucinous, undiff./[Inv] 96 (73,3)/[61] 171 (81,4)[110] FIGO stage (all/invasive cases)* I, n (%)/ 70 (53,4)/37(38,9)* 84(40)/32(22,4) II, n (%)/ 8(6,1)/6(6,3) 25(11,9)/17(11,9) III, n (%)/ 38(29,0)/37(38,9)* 79(37,6)/73(51,0) IV, n (%)/ 15(11,5)/15(15,8) 22(10,5)/21(14,7) Survival at 5 years (all/inv) • Operated <5 years 26(19,8)/17(17,9) 50(23,8)/30(21,0) • Dead 5 years 52(39,7)/32(33,7) + 64(30,5)/34(23,8) • Unknown. 19(14,5)/14(14,7) 26(12,4)/13(9,1) *chi-squared test 0,000; +, chi-squared test 0,066 (NS). n = number; m = mean; SD = standard deviation; (r)=range; %= percentage; BSR: blood sedimentation rate; NS: not statistically significant. Baseline characteristics of studied patients with ovarian epithelial tumors. Operated <5 years Dead 5 years Unknown. *chi-squared test 0,000; +, chi-squared test 0,066 (NS). n = number; m = mean; SD = standard deviation; (r)=range; %= percentage; BSR: blood sedimentation rate; NS: not statistically significant. Table 2 shows the differential clinical features of OETs associated (n = 36) or not associated (n = 305) with endometriosis. The most important of these differential features were significantly lower age (50.9 years versus 56.7), parity (50% nulliparous versus 26.6%), and premenopausal status (55.6% versus 33.8%). There were no significant differences in personal or family history of cancer or endometriosis. Moreover, endometriosis was classed as atypical or with p53+ in eight of the 36 cases (22.2%). With regard to the analytical data, the blood sedimentation rate was similar and tumor markers carbohydrate antigen (CA)-125 and CA-19-9 were higher in patients with OETs without endometriosis, but the differences did not reach statistical significance. Neither the differences for the presence of ascites nor for the surgery performed were significant, but it was significant that the percentage of cytoreduction was optimal in cases with associated endometriosis (94.3% versus 68.6% in OETs without endometriosis). This pattern was the same for International Federation of Gynecology and Obstetrics (FIGO) cancer stage, which was significantly lower in those with endometriosis-associated OET (63.9% were FIGO stage I versus 43% in OETs without endometriosis; and 16.7% were FIGO stage III versus 36.1% in OETs without endometriosis). Table 2 Differential clinical features between ovarian epithelial tumors (OETs) associated and not associated with endometriosis. Table 2 OET with endometriosis OET without endometriosis RR (CI) P, Chi 2 Number of cases 36 305 – Age, years [n] m ± SD (r) [36] 50.9 ± 11.2 (32-76) [305] 56.7 ± 14.9 (16-87) <0.025 Parity, (n, %) [36] 1.03±1.23 [305] 1.74±1.49 <0.005  Nulliparous 18 (50) 81 (26.6) 1,5(1,05-2,05  ≥Primiparous 18 (50) 224 (73.4) NS Premenopausal, (n, %) 20 (55.6) 103 (33.8) Postmenopausal, (n, %) 16 (44.4) 201 (65.9) <0.035 Age of menopause, years [n] m ± SD [16] 50.3 ± 4.6 [153] 48.8 ± 4.2 NS Personal history (n, %) • No history 22 (61.1) [121] 86 (71.1) NS • Breast cancer 1 (2.8) 2 (1.7) • Endometrial cancer 0 (0) 1 (0.8) • Endometriosis 2 (5.6) 4 (3.3) • Other 11 (30.5) 28 (23.2) Family history (n, %) • No history 28 (77.8) [124] 82 (66.1) NS • Ovarian cancer 1 (2.8) 2 (1.6) • Endometrial cancer 0 (0) 6 (4.8) • Breast cancer 2 (5.6) 10 (8.1) • Other 5 (13.8) 24 (19.5) CA-125 U/mL [n, m ± SD] (29) 224.9 ± 311.18 (192) 625.6 ± 1314.3 NS CA-19−9 U/mL [n, m ± SD] (28) 244.7 ± 484.6 (185) 525.7 ± 3836.6 NS BSR mm/h [n, m ± SD] (26) 41.50 ± 34.6 (154) 34.9 ± 27.8 NS Ascites (n, %)  Abundant [23]2 (8.7) [113]27 (23.9) NS NS  Mild 3 (13.0) 16 (14.2)  No ascites 18 (78.3) 70 (61.9) Surgery (n, %)  Conservative surgery: laparotomy 3 (8.3) [282] 54 (19.1) NS  Conservative surgery: laparoscopy 2 (5.6) 9 (3.2)  Hysterectomy + bilateral adnexectomy 3 (8.3) 29 (10.3)  Extended surgery 28 (77.8) 190 (67.4) – Cytoreduction, (n, %)  Optimal 34 (94.3) [121] 83 (68.6) 0,7 (0,6-0,8) 0,008  >1 cm remaining 2 (5.7) 24 (19.8)  Biopsy only and closure 0 (0) 14 (11.6) FIGO stage (n, %)  I 23 (63.9) 131 (43.0) 0,7(0,5-0,9) 0,07  II 6 (16.7) 27 (8.9) 2,2(1,02-4,56)  III 6 (16.7) 110 (36.1)  IV 0 (0) 37 (12.1) [n]=number; m = mean; SD = standard deviation; (r)=range; %= percentage; RR: relative risk; CI: confidence interval; BSR: blood sedimentation rate; FIGO: International Federation of Gynecology and Obstetrics; NS: not statistically significant. Differential clinical features between ovarian epithelial tumors (OETs) associated and not associated with endometriosis. No history Breast cancer Endometrial cancer Endometriosis Other No history Ovarian cancer Endometrial cancer Breast cancer Other [n]=number; m = mean; SD = standard deviation; (r)=range; %= percentage; RR: relative risk; CI: confidence interval; BSR: blood sedimentation rate; FIGO: International Federation of Gynecology and Obstetrics; NS: not statistically significant. Histological subtypes, invasiveness, recurrence, and survival were also compared between OETs with and without endometriosis. As shown in Table 3 , the total percentage of endometrioid and clear cell OETs, both individually and jointly, was greater in the endometriosis-associated OET group (75% versus 15.4%), while the opposite was observed for serous, mucinous, and undifferentiated OET. A statistically significant association was found between endometriosis and an increased risk of endometrioid and clear cell OET versus OET without endometriosis (RR 6.4; 95% CI, 4–10.5 and RR 4.2; 95% CI, 2–9.2 for endometrioid and clear cells OET respectively). However, most serous and mucinous tumors were not associated with endometriosis; when they were, they were more often borderline tumors. Differences in type I Kurman and Shih’s classifications were also significant (97% versus 46%; Chi-squared test, 0.000) Table 3 Comparisons of ovarian epithelial tumors (OETs) associated or not associated with endometriosis according to histological type, invasiveness, recurrence, and survival. Table 3 OET with Endometriosis (36) OET without Endometriosis (305) RR (CI) P Chi 2 Histological Subtype (n, %) Serous 3 (8.3) 146 (47.9) 0.17 (BL=51; Inv=98) (2 BL, 1 Inv) (49 BL, 97 Inv) 0.05-0.5) Mucinous 6 (16.7) 69 (22.6) NS (BL=45; Inv=30) (6 BL) (39 BL, 30 Inv) Endometrioid 19 (52.8) 25 (8.2) 6.4 (BL=6; Inv=38) (5 BL, 14 Inv) (1 BL, 24 Inv) (4-10.5) Clear cell 8 (22.2) 16 (5.2) 4.2 (BL=1; Inv=23) (8 Inv) (1 BL, 15 Inv) (2-9.2) Endometrioid + clear 27 (75.0) 47 (15.4) 4.9 cell+mix (5 BL, 22 Inv) (2 BL, 45 Inv) (3.5-6.7) Undifferentiated 0(0) 43 (14.1) -- (0) (43 Inv) Borderline (n, %) 13 (36.1) 90 (29.5) NS Invasive (n, %) 23 (63.9) 215 (70.5) Kurman and Shih’s classification (n, %)  Type I 35 (97.2) 165 (54.1) 1.8 <0.000  Type II 1 (2.8) 140 (45.9) (1.6-2.02) No. of recurrences, in months [2] {5.2} 15 ± 12.72; (6-24) [19] {16.4} 20.68 ± 13.35; (1-47) NS [n] {%} m ± SD (r) Associated endometrial cancer, (n, %) 6 (16.8) 15 (4.9) 3.4 (1.4-8.2) Survival in BL/Inv and total, n (%) at:  >2 years 9 (69.2)/13 ( 56.5 ), 68 (75.6)/104(48.4), NS 0,027 22 (61.1) 172 (56.4)  >5 years 3(23.1)/8( 34.8 ), 47(52.2)/58(27.0), NS 0,006 11(30.6) 105(34.4)  >10 years 1(7.7)/5( 21.7 ), 19(21.1)/28(13.0), NS 0,039 6(16.7) 47(15.4) Kaplan–Meier survival (%)  2 years (BL/Inv) (100/ 84,6 ) (97,4 + /64,4 x ) 0,8 (0,6-0,9)  5 years (BL/Inv) (84,6/ 76,9 ) (92,4 + /46,6 x ) 0,6 (0,5-0,8)  10 years (BL/Inv) (84,6/ 65,9 ) (90,1 + /33,6 x ) 0,5 (0,4-0,6) (n)=number; m = mean; SD = standard deviation; (r)=range; %= percentage; RR = relative risk; CI = confidence interval; BL = borderline; Inv = invasive; NS = not statistically significant. +, RR, not significant (BL) / x, RR, significant (Inv). Comparisons of ovarian epithelial tumors (OETs) associated or not associated with endometriosis according to histological type, invasiveness, recurrence, and survival. (n)=number; m = mean; SD = standard deviation; (r)=range; %= percentage; RR = relative risk; CI = confidence interval; BL = borderline; Inv = invasive; NS = not statistically significant. +, RR, not significant (BL) / x, RR, significant (Inv). There were no significant differences in recurrences due to the low number of cases considered. Likewise, the finding of synchronous endometrial cancer and OET was not significant, but there was an increased risk of this association (RR 3.4; 95% CI, 1.4–8.2) in the endometriosis-associated OET group, although endometrioid was the only subtype. We observed increased survival (significant for invasive tumors) in the endometriosis-associated OET group for all assessed time intervals (two, five, and 10 years). As shown in Fig. 2 A, comparison of the survival rates for OETs with and without endometriosis and according to borderline or invasive status revealed worse two-, five-, and 10-year survival rates for invasive tumors without associated endometriosis. Patients with borderline tumors survived longer than those who presented with an invasive tumor but women with invasive tumors that were not associated with endometriosis had a lower survival rate than those with associated endometriosis (p < 0.05). As shown in Fig. 2 B, analysis and representation of two-, five-, and 10-year survival by Kaplan-Meyer analysis in invasive OETs with or without associated endometriosis according to histological subtype revealed lower survival rates in cases without associated endometriosis and undifferentiated histology, mucinous, clear cell and serous (in this same reverse order) and the best survival rates in clear cell and endometrioid with endometriosis. Finally, Fig. 2 C also shows the analysis and representation of two-, five-, and 10-year survival by Kaplan-Meyer analysis in invasive OET with or without associated endometriosis according to FIGO stages. Survival was greater than 90% at 10 years in stage I and II with associated endometriosis; between 60% and 80% in stages I and II without associated endometriosis; and less than 30% in stage III and IV (usually without associated endometriosis). Fig. 2 Kaplan-Meier survival curves for ovarian epithelial tumors at two, five, and 10 years. A) In borderline and invasive ovarian epithelial tumors with or without endometriosis. B) In invasive ovarian epithelial tumors (according to histological subtype) with and without endometriosis. C. In invasive OET (according to FIGO stage) with and without endometriosis. Fig. 2 Kaplan-Meier survival curves for ovarian epithelial tumors at two, five, and 10 years. A) In borderline and invasive ovarian epithelial tumors with or without endometriosis. B) In invasive ovarian epithelial tumors (according to histological subtype) with and without endometriosis. C. In invasive OET (according to FIGO stage) with and without endometriosis. Table 4 shows the immunohistochemical results in patients with endometriosis-associated endometrioid (n = 7) or clear cell (n = 6) OET. The endometrioid subtypes presented estrogen-receptor, progesterone-receptor, and p53 positivity, in both the endometriotic tissue and the tumor. However, in the clear cell subtype, estrogen-receptor and progesterone-receptor positivity was observed in the endometriotic tissue and not in the tumor, a statistically significant difference. The p53 protein was present in both the endometriotic tissue and the tumor, especially in clear cell OET. Table 4 Immunohistochemistry (ER, PR, and p53) of patients with endometrioid versus clear cell ovarian epithelial tumors (OETs) with associated endometriosis at Hospital Marina Baixa (HMB). Table 4 IHC Endometrioid OET (n, %) Clear cell OET (n, %) P-value Endometriosis ER(+) 4/4 (100) 2/2 (100) NS PR(+) 4/4 (100) 1/2 (50) NS P53(+) 2/4 (50) 2/2 (100) NS Tumor ER(+) 6/7 ( 85.7 ) 0/6 (0) <0.05 PR(+) 6/7 ( 85.7 ) 1/6 (16.6) <0.05 P53(+) 5/7 (71.4) 5/6 (83.4) NS (n)=number; %= percentage; IHC = immunohistochemistry; ER = estrogen receptor, PR = progesterone receptor; NS = not statistically significant. Immunohistochemistry (ER, PR, and p53) of patients with endometrioid versus clear cell ovarian epithelial tumors (OETs) with associated endometriosis at Hospital Marina Baixa (HMB). (n)=number; %= percentage; IHC = immunohistochemistry; ER = estrogen receptor, PR = progesterone receptor; NS = not statistically significant. Finally, Table 5 shows two cases with endometriosis-associated OET but with histologically confirmed previous endometriosis whose progress was monitored. Case 1 (endometrioid OET and endometriosis) had previously been diagnosed with atypical endometriosis and was estrogen-receptor (-), progesterone-receptor (-), and p53-positive; CA-19-9, in particular, was increased when she suffered a recurrence and the histopathology of the second operation once again showed endometriosis together with a borderline endometrioid tumor, with positive staining for estrogen-receptor, progesterone-receptor, and aromatase in the tumor and endometriotic tissues. P53 protein positivity was observed in tumor but not in endometriotic tissue. After her endometriosis diagnosis, Case 2 (clear cell carcinoma and endometriosis) was followed-up for infertility and underwent in vitro fertilization and a twin pregnancy. Three years later, she required urgent hospital admission for a superinfected ovarian cyst or endometrioma and hemorrhagic ascites. Her blood sedimentation rate, CA-125, and CA-19-9 values were all elevated, particularly CA-125. Surgery and histopathology showed undifferentiated clear cell carcinoma and foci of endometriosis throughout the pelvis. Immunohistochemical study showed positive staining for p53 protein in the endometriosis and tumor and estrogen-receptor and progesterone-receptor positivity in the endometriotic tissue but not in the tumor. Table 5 Cases of ovarian epithelial tumors (OETs) with histologically confirmed previous endometriosis. Table 5 Case 1 (Endometrioid) Case 2 (Clear cell) Years since initial surgical diagnosis 2 9 Primary infertility Yes Yes Histopathology in first surgery Atypical endometriosis Endometriosis Immunohistochemistry in first surgery ER+, PR+, p53+ Unknown Subsequent pregnancy No Yes, IVF, twin pregnancy Episode of recurrence Frequent No. Stable endometrioma of the right ovary. Last follow-up visit 3 years earlier CT/Transvaginal ultrasound Bilateral endometrioma Heterogeneous mass suspicious of infected endometrioma or abscess or intracystic hemorrhage or hemorrhagic ascites?? Hb 11.8 – 11.9 g/dL 9.7 – 9.3 – 9.1 g/dL CRP Unknown 4.16 – 15.8 – 23.6 mg/dL BSR 10 - 18 mm/h 71 mm/h CA-125 71 U/mL 489 U/mL CA-19-9 442 U/mL 104 U/mL Surgery Conservative Radical Age 32 45 Histopathology Right ovary: atypical endometriosis + borderline endometrioid tumor Undifferentiated clear cell carcinoma in the right ovary + foci of endometriosis throughout the pelvis. Left ovary: endometrial cyst p53 in endometriosis Negative Positive (+) p53 in the tumor Positive (++) Positive (+) ER and PR in endometriosis Positive (+++) Positive (++) ER and PR in the tumor Positive (+++) Negative Aromatase Positive Unknown Recurrence Yes, after GnRH analogues + anastrozole No, chemotherapy ER = estrogen receptor; PR = progesterone receptor; IVF = in vitro fertilization; CT = computed tomography; Hb = hemoglobin; BSR = blood sedimentation rate; CRP = C-reactive protein. Cases of ovarian epithelial tumors (OETs) with histologically confirmed previous endometriosis. ER = estrogen receptor; PR = progesterone receptor; IVF = in vitro fertilization; CT = computed tomography; Hb = hemoglobin; BSR = blood sedimentation rate; CRP = C-reactive protein.

No conflict of interest.

This retrospective study analyzed clinical and pathological data from operated patients having borderline or invasive OET with associated endometriosis compared to those of other cases also operated for OET without associated endometriosis. Two series of patients were included in this study. Series 1 included all 131 cases of borderline (n = 36) and invasive or malignant (n = 95) diagnosed OETs operated and treated at Hospital Marina Baixa between June 1995 and June 2015. Review of the histopathological reports of all cases revealed that 15 (11.5%) patients had associated endometriosis. Series 2 included all 210 cases of borderline (n = 67) and invasive or malignant (n = 143) diagnosed OETs operated and treated at San Juan University Hospital during the same period, including 21 (10%) cases of OET with associated endometriosis. Therefore, this study included and analyzed data from 341 patients with OETs: 36 cases of endometriosis-associated OET (13 borderline and 23 invasive) and 305 without associated endometriosis, including 90 and 215 cases of borderline and invasive OETs, respectively. The epidemiological, clinical, histopathological, and survival data of the 341 included patients were revised, studied, and analyzed. The borderline OETs (103 cases, 302%, 13 with associated endometriosis and 90 without endometriosis) included serous, mucinous, endometrioid, and clear cell or mesonephroid tumors with marginal malignancy or low malignant potential according to World Health Organization (WHO) histological classification [ 22 ]. The invasive or malignant OETs (238 cases, 69,8%, 23 with and 215 without endometriosis, respectively) included serous, mucinous, endometrioid, clear cell or mesonephroid, and mixed malignant, as well as undifferentiated carcinomas. Patient clinical characteristics, tumor markers, tumor histology and staging, associated pathology, surgery performed, postoperative clinical course, and data for survival analysis were collected. Data on the presence and type of endometriosis when associated with OET, as well as the association with other malignant tumors, especially endometrial carcinoma, were also collected. In this study, endometriosis-associated ovarian carcinoma was defined by the presence of OET and endometriosis in the same or contralateral ovary or extraovarian pelvic endometriosis. Endometriosis was identified as tissue resembling endometrial stroma surrounding epithelial glands present in the ovaries or peritoneum. Atypical endometriosis was defined according to the criteria proposed by Thomas and Campbell [ 23 ] based on features identified in histological examinations as well as positivity for p53 expression. According to these criteria, atypical endometriosis was identified in postoperative pathological study of five cases associated with OET (all from series 2) and 3 of 15 patients in series 1 were possibly atypical (p53+). Furthermore, based on a dualistic model of carcinogenesis and Kurman and Shih’s classification [ 24 , 25 ], we divided the OET cases included in this study into two groups: 1) type I tumors comprising borderline and low-grade serous carcinomas, mucinous, endometrioid, clear cell, and transitional cell carcinomas, and 2) type II tumors comprising high-grade serous cancer, malignant mixed mesodermal tumors, and undifferentiated carcinomas. The clinical and pathological characteristics as well as the survival (%) at two, five and 10 years and the actuarial survival (Kaplan-Meier method) of the different subtypes, with or without associated endometriosis, were compared. Immunohistochemical studies were also performed in cases with endometriosis-associated endometrioid and clear cell OETs from series 1, to analyze any differences in the presence or absence of estrogen-receptor, progesterone-receptor, and p53 protein expression both in endometriosis and in the tumor (see Fig. 1 ). Finally, we individually analyzed and described two cases (from series 2) with histologically confirmed endometriosis prior to OET diagnosis and with data on previous and subsequent evolution. Fig. 1 Immunohistochemical study for estrogen and progesterone receptors and p53 protein. A) Endometrioid carcinoma: moderate positivity for estrogen receptors, 40×. B) Endometrioid carcinoma: moderate-intense positivity for progesterone receptors, 40×. C) Endometriosis: moderate-intense positivity for p53, 40×. Fig. 1 Immunohistochemical study for estrogen and progesterone receptors and p53 protein. A) Endometrioid carcinoma: moderate positivity for estrogen receptors, 40×. B) Endometrioid carcinoma: moderate-intense positivity for progesterone receptors, 40×. C) Endometriosis: moderate-intense positivity for p53, 40×. The statistical analysis was performed as follows: qualitative data were analyzed in terms of frequencies and percentages and quantitative data in terms of means and standard deviation (SD). Comparison of independent means (Student’s t-test), comparison of two proportions (relative risk [RR]), 95% confidence intervals (CIs), and Chi-square tests were used to compare groups. Finally, the actuarial survival was analyzed in all OET groups after two, five, and 10 years of follow-up according to the Kaplan-Meier method of estimated survival. Statistical treatment of the data was performed using IBM SPSS Statistics, version 20.0 and 25 (IBM-Spain), CIcalculator, and Rsigma (Systat Software, San Jose, Calif., USA). All p-values reported were 2-tailed and p < 0.05 was considered statistically significant.

Ovarian epithelial tumors (OETs) represent 75% of all ovarian tumors and 90% of borderline or malignant tumors in this location [ 1 ]. OETs originate in the epithelium that lines the ovary, which in turn is embryologically derived from the primitive peritoneum. The Müllerian ducts also derive from an invagination of the primitive peritoneum that results in the Fallopian tubes and uterus. OETs are, therefore, similar to tumors arising from the Müllerian duct in that they may be tubes (serous), endocervical (mucinous), endometrial (endometrioid and clear cell, and may undergo urothelial metaplasia (transitional cell tumors) [ 1 ]. Endometrioid and clear cell OETs are often associated with endometriosis [ 2 ], which has been suggested to be the origin of this type of tumor [ 3 ]. Atypical endometriosis [ 4 ] has also been suggested as a transitional phase from benign endometriosis to carcinoma, meaning that it could be a precursor lesion of endometrioid and clear cell ovarian cancers [ 3 , [5] , [6] , [7] ]. Ovarian cancers that are associated with endometriosis appear to be a different entity, with an earlier age of onset and a relatively better prognosis [ [8] , [9] , [10] ]; therefore, concurrent endometriosis could be a factor of good prognosis for associated ovarian cancers [ [11] , [12] , [13] ]. Sampson [ 14 ] was the first to describe the association between endometriosis and carcinoma, later followed by Scott [ 15 ], who defined the diagnosis of endometriosis-associated OET. Since then, numerous studies have analyzed this association [ [16] , [17] , [18] , [19] ]. Wei et al. [ 3 ] also reviewed the natural history of endometriosis and its possible progression to endometriosis-associated OET. Some cases of endometriosis-associated OETs seem to be the ultimate consequence of such processes. However, in most patients with associated endometriosis, this progression and malignant transformation are not so clear, as the finding of endometriosis associated with OET is synchronous in most cases and there is not enough evidence of patients with histologically confirmed endometriosis who have subsequently developed carcinoma [ 20 , 21 ]. The objectives of this study were to 1) analyze cases of endometriosis-associated borderline or malignant OET to assess their differential features in comparison with cases without associated endometriosis and factors that may influence the association or progression to carcinoma, and 2) to deduce from previous analyses whether the clinical, histopathological, and immunohistochemical findings support the possible progression of endometriosis to endometriosis-associated carcinoma and, ultimately, to ovarian cancer.