Effect of IL-Iβ and TNF-α on the expression of monocyte chemotactic protein-1 in endometriotic cells

Summary To investigate the clinical significance of monocyte chemotactic protein-1 (MCP-1) produced by endometriotic tissues, the endometriotic tissues were taken from 15 patients with endometriosis. MCP-1 mRNA and MCP-1 protein were determined by dot blot analysis and enzyme linked immunosorbent assay (ELISA) in endometriotic cells cultured with or without interleukin-1β (IL-lβ, 2 μg/L), tumor necrosis factor-α (TNF-α, 20 g/L). After exposure to IL-1β or TNF-α, the expression of MCP-1 mRNA in the endometriotic cells (8.635 ±0.826, 7.031 ±0.970, respectively) were significantly higher than that in the control group (4.482±0.435, P<0.05); The expression of MCP-1 protein in IL-lβ and TNF-α group was 4.52±0.09 μg/L,2.87±0.27 μg/L, respectively, which were significantly higher than 1.74±0.16 μg/L in control (P<0.01). The results suggested that IL-l\ and TNF-α could up-regulate the expression of MCP-1 in endometriotic cells, which might be related to the development of endometriosis. Similar content being viewed by others References Weil S J, Wang S, Perez M C. Chemotaxis of macrophages by a peritoneal fluid of patients with endometriosis. Fertil Steril, 1997, 67:865 Dodds W, Miller F, Feidman Cet al. The effect of preovulatory peritoneal fluid from cases of endometriosis on murinein vitro fertilization, enbryo development, oviduct transport and implantation. Am J Obstet Gynecol, 1992, 166:219 Taketani Y, Kuo T M, Mizuno M. Comparison of cytokine levels and embryo toxicity in peritoneal fluid in infertil women with untreated and treated endometriosis. Am J Obstet Gynecol, 1992, 67:765 Randolph G J, Furie M B. A soluble gradient of endogenous monocyte chemoattractant protein-1 promotes the transendothelial migration of monocytes in vitro. J Immunol, 1995, 155:3610 Yoshimera T, Leonard E J. Identification of high affinity receptors for human monocyte chemoattractant protein-1 on human monocyte. J Immunol, 1990, 145:292 Jolicoeur C, Boutouil I. Increased expression of monocyte chemotactic protein-1 in the endometrium of woman with endometriosis. Am J Pathol, 1998, 152:125 Author information Authors and Affiliations Rights and permissions About this article Cite this article Ying, G., Lilan, L. & Fuxian, H. Effect of IL-Iβ and TNF-α on the expression of monocyte chemotactic protein-1 in endometriotic cells. Current Medical Science 19, 212–214 (1999). https://doi.org/10.1007/BF02887737 Received: Published: Issue date: DOI: https://doi.org/10.1007/BF02887737