Stromal Heterogeneity in the Human Proliferative Endometrium—A Single-Cell RNA Sequencing Study

Suzanna Queckbörner, Carolina von Grothusen, Nageswara Rao Boggavarapu, Roy Mathew Francis, Lindsay C Davies, Kristina Gemzell‐Danielsson
Journal of personalized medicine · 2021 · vol. 11(6) , pp. 448 · doi:10.3390/jpm11060448 · PMID:34067358 · W3165705240
article OA: gold CC0 ⤵ 22 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-08 ⓘ

Single-cell RNA sequencing of human proliferative endometrium identified diverse stromal cell populations and pericyte subsets, revealing distinct niches potentially regulating inflammation and extracellular matrix.

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Abstract

The endometrium undergoes regular regeneration and stromal proliferation as part of the normal menstrual cycle. To better understand cellular interactions driving the mechanisms in endometrial regeneration we employed single-cell RNA sequencing. Endometrial biopsies were obtained during the proliferative phase of the menstrual cycle from healthy fertile women and processed to single-cell suspensions which were submitted for sequencing. In addition to known endometrial cell types, bioinformatic analysis revealed multiple stromal populations suggestive of specific stromal niches with the ability to control inflammation and extracellular matrix composition. Ten different stromal cells and two pericyte subsets were identified. Applying different R packages (Seurat, SingleR, Velocyto) we established cell cluster diversity and cell lineage/trajectory, while using external data to validate our findings. By understanding healthy regeneration in the described stromal compartments, we aim to identify points of further investigation and possible targets for novel therapy development for benign gynecological disorders affecting endometrial regeneration and proliferation such as endometriosis and Asherman's syndrome.

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endometriosis

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europepmc
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