The Relationship between Functional Promoter Variants of Macrophage Migration Inhibitory Factor and Endometriosis.

Zahra Chekini, Maryam Shahhoseini, Reza Aflatoonian, Parvaneh Afsharian
Cell journal · 2021 · vol. 22(4) , pp. 450–456 · doi:10.22074/cellj.2021.6858 · PMID:32347038 · W3019882361
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This study investigated the relationship between functional promoter variants of macrophage migration inhibitory factor (MIF) and endometriosis, finding a specific promoter haplotype associated with increased MIF expression in ectopic endometrial tissues.

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This 2015–2017 case-control study evaluated whether macrophage migration inhibitory factor (MIF) promoter polymorphisms (−794 CATT repeats and −173G/C) were associated with susceptibility to endometriosis and whether they correlated with MIF mRNA levels in ectopic endometrial tissues. Researchers genotyped 106 endometriosis patients and 110 controls, and performed gene expression analyses in 17 endometrioma tissues during the secretory phase using RFLP/sequencing and qPCR. They found no significant differences in allele and genotype frequencies between patients and controls, but reported that certain haplotype patterns differed, and that ectopic-tissue mRNA levels were significantly higher in endometriosis patients with CATT6,7/CC haplotypes versus several other haplotypes. The paper’s conclusions are limited by the lack of significant single-variant differences and the small ectopic-tissue sample size. This paper is centrally about endometriosis — it tests associations between MIF promoter variants, haplotypes, and MIF expression in endometriosis patients.

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Abstract

OBJECTIVE: promoter polymorphisms and susceptibly to endometriosis and its corolation with mRNA level. MATERIALS AND METHODS: were detected by sequencing. Quantitative polymerase chain reaction (Q-PCR) was carried out to determine MIF expression level. RESULTS: /GG (2.08 fold, P=0.046). CONCLUSION: promoter in ectopic endometrial tissues. This promoter haplotype might play an important role in the development and establishment of endometriosis.
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Cell Journal (Jan 2021) The Relationship Between Functional Promoter Variants Of Macrophage Migration Inhibitory Factor And Endometriosis Abstract Objective: Endometriosis is a common gynecological and inflammatory disorder. Macrophage migration inhibitory factor (MIF) is a key pro-inflammatory cytokine that is secreted by accumulated active macrophages in ectopic endometrial tissues. Two promoter polymorphisms of MIF [-794(CATT)5–8 /-173G/C] were identified to susceptibility and severity of several immune and inflammatory diseases. We aimed to evaluate the possible association between MIF promoter polymorphisms and susceptibly to endometriosis and its corolation with mRNA level. Materials and Methods: This case-control study was performed in Royan Institute from 2015 to 2017. Polymorphisms were evaluated in 106 endometriosis patients and 110 controls. For 17 endometrioma tissues, gene expression studies were conducted during secretory phase of menstrual cycle. Restriction fragment length polymorphism (RFLP) analysis was performed to determine -173G/C polymorphism and -794(CATT)5-8 were detected by sequencing. Quantitative polymerase chain reaction (Q-PCR) was carried out to determine MIF expression level. Results: Homozygote of CATT7 was observed only in endometriosis whilst we did not detect the significant allele and genotype variation in both groups. The homozygotes for -794(CATT)5–8 and -173G/C polymorphisms were obtained to estimate the haplotype frequencies. Significantly higher haplotype frequencies were observed for CATT5/G in controls [global P value=0.044]. Additionally, the CATT5/C and CATT7/G haplotypes were not detected in any groups. Expression level of mRNA in ectopic tissue of endometriosis patients with CATT6,7/CC haplotype, were significantly higher compared to other haplotypes including CATT5,5/GG (2.91 fold, P=0.007), CATT5,5/GC (2.48 fold, P=0.047) and CATT6,6/GG (2.08 fold, P=0.046). Conclusion: We report, for the first time, a strong linkage between the decreased repetition of CATT and G allele in control and CATT6/C and CATT7/C haplotypes in endometriosis patients. Increased MIF expression is affected by genetic variants in the MIF promoter in ectopic endometrial tissues. This promoter haplotype might play an important role in the development and establishment of endometriosis. Keywords

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endometriosis

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