High ovarian response to ovarian stimulation: effect on morphokinetic milestones and cycle outcomes.

Purpose To assess the effect of high ovarian response on oocyte quality and ovarian stimulation cycle outcomes.

A retrospective cohort study conducted at three IVF units. The high ovarian response (HOR) and polycystic ovary syndrome (PCOS) with HOR (PCOS HOR) groups included 151 and 13 women who underwent controlled ovarian stimulation (COS) resulting in more than 15 retrieved oocytes, for a total of 1863 and 116 cultured embryos, respectively. The normal ovarian response (NOR) group comprised 741 women with 6–15 retrieved oocytes, resulting in 4907 cultured embryos. Data collected included fresh cycle data and pregnancy rates, in addition to annotation of morphokinetic events from time of pronuclei fading to time of initiation of blastocyst formation of embryos cultured in a time lapse incubator, including occurrence of direct unequal cleavage at first cleavage (DUC-1) (less than 5 h from two to three blastomeres). Comparison was made between morphokinetic parameters between the 3 groups. Cycle outcomes were compared in the high vs. normal ovarian response groups.

Oocyte maturation rate was significantly lower in the HOR vs. NOR groups (56.5% vs. 90.0%, p < 0.001), while the fertilization rates were similar (60.2% vs. 58.1%, p = 0.397). The prevalence of DUC-1 embryos was higher in the PCOS HOR and the HOR groups as compared to the NOR group (22.7% vs. 16.2% and 12.0%, respectively, p < 0.001). After exclusion of DUC-1 embryos, remaining embryos from the NOR and HOR groups reached the morphokinetic milestones at similar rates, with comparable implantation and clinical pregnancy rates, while the PCOS HOR showed shorter time to 5 blastomeres compared to the NOR and HOR groups.

High ovarian response might be associated with decreased oocyte quality, manifested as a higher proportion of immature oocytes and higher rate of direct uneven cleavage embryos, while embryos exhibiting normal first cleavage have similar temporal milestones and implantation potential. Access this article We’re sorry, something doesn't seem to be working properly. Please try refreshing the page. If that doesn't work, please contact support so we can address the problem. Similar content being viewed by others Data availability Available upon request.

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Author information Authors and Affiliations Contributions Natali Schachter-Safrai and Gilad Karavani have contributed substantially to the conception and design of the study, analysis and interpretation of data, and drafting the article. Efrat Esh-Broder and Eliahu Levitas have contributed substantially to the acquisition, analysis and interpretation of data, and revision of the article. Tamar Wainstock has contributed substantially to the conception and design of the study, analysis and interpretation of data, and revision of the article. Iris Har-Vardi and Assaf Ben-Meir have contributed substantially to the conception and design of the study, acquisition, analysis and interpretation of data, and drafting and revision of the article. All authors have approved the final version of the study. Corresponding author Ethics declarations Conflicts of interest The authors declare that they have no conflict of interest. Additional information Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Natali Schachter-Safrai and Gilad Karavani should be considered as equal contribution first authors. Iris Har-Vardi and Assaf Ben-Meir should be considered as equal contribution last authors. Rights and permissions About this article Cite this article Schachter-Safrai, N., Karavani, G., Esh-Broder, E. et al. High ovarian response to ovarian stimulation: effect on morphokinetic milestones and cycle outcomes. J Assist Reprod Genet 38, 3083–3090 (2021). https://doi.org/10.1007/s10815-021-02323-w Received: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s10815-021-02323-w