[AQP5 gene silencing inhibits proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis].

Liao-bing Xin, Xin LB, Liaobing Xin, Jiang XX, Xiuxiu Jiang, Xiu-xiu Jiang, Ye XL, Xiao-lei Ye, Ruijin Wu, Wu RJ, Rui-Jin Wu, Xu KH, Kaihong Xu, Kai-Hong Xu, Jun-Yan Ma, Junyan Ma, Ma JY, Jun Lin, Lin J
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2015 · vol. 44(3) , pp. 285–92 · doi:10.3785/j.issn.1008-9292.2015.05.08 · PMID:26350009 · PMC10397035 · W1624656187
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⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-08 ⓘ

Silencing the AQP5 gene inhibited proliferation and migration of ectopic endometrial epithelial cells and nodule formation in nude mice, potentially via the AKT pathway.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-08 · read from full text ⓘ

The study investigated whether silencing the AQP5 gene affects the behavior of ectopic endometrial glandular epithelial cells associated with endometriosis. Using AQP5 gene knockdown in cultured ectopic glandular epithelial cells, the authors reported inhibition of both cell proliferation and cell migration. The main caveat is that the work appears to focus on in vitro cellular experiments rather than in vivo validation or clinical correlation. This paper is centrally about endometriosis — specifically, it tests how AQP5 gene silencing impacts proliferation and migration of ectopic endometrial glandular epithelial cells in endometriosis.

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Abstract

OBJECTIVE: To investigate the effect of aquaporin 5(AQP5) on proliferation and migration of ectopic endometrial epithelial cells. METHODS: AQP5 shRNA interference fragments were designed and transfected into ectopic endometrial epithelial cells stably by lentivirus technology. Fluorescence quantitative RT-PCR and Western blotting were used to detect the AQP5 mRNA and protein expression, respectively. The cell proliferation and migration were determined by using MTT method and Transwell system, respectively. Levels of phosphorylated AKT(p-AKT) and total AKT were examined by Western blotting. The nude mice model of endometriosis was constructed and the endometrial cell nodule formation was observed. RESULTS: AQP5 shRNA transfection inhibited cell proliferation and migration compared with control group (both P<0.05). The activation of AKT in AQP5 shRNA transfected cells was lower than that in control cells (P<0.01). Compared to control group, the endometrial cells nodule formation was suppressed in mice inoculated with AQP5 shRNA-silencing ectopic endometrial epithelial cells. CONCLUSION: Down-regulation of AQP5 expression can suppress the proliferation and migration of ectopic endometrial epithelial cells and endometrial cell nodule formation in nude mice, in which AKT pathway may be involved.
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Condition tags

endometriosis

MeSH descriptors

Aquaporin 5 Cell Movement Cell Proliferation Endometriosis Epithelial Cells Gene Silencing Animals Aquaporin 5 Disease Models, Animal Down-Regulation Endometriosis Epithelial Cells Female Mice Mice, Nude Phosphorylation Proto-Oncogene Proteins c-akt Proto-Oncogene Proteins c-akt RNA, Messenger RNA, Small Interfering

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