Rapid Effects of Oestrogen on Intracellular Ca2+ in the Uterine Junctional Myometrium of Patients With and Without Adenomyosis in Different Phases of the Menstrual Cycle

Sha Wang, Hua Duan, Bohan Li
Reproductive sciences (Thousand Oaks, Calif.) · 2020 · vol. 27(11) , pp. 1992–2001 · doi:10.1007/s43032-020-00218-2 · PMID:32542538 · W3034366256
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⚙ AI-generated summary by claude@2026-06+body, 2026-06-07 ⓘ

Oestrogen rapidly increases intracellular calcium in uterine junctional myometrial cells via ESR1 and the phospholipase C pathway, with altered levels found in adenomyosis.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study examined how rapid, non-genomic estrogen signaling through estrogen receptor 1 (ESR1) regulates intracellular calcium concentrations ([Ca2+]i) in uterine junctional zone smooth muscle cells (JZSMCs) isolated from 17 controls and 24 patients with adenomyosis, analyzing differences across proliferative versus secretory menstrual phases. Membrane ESR1 and [Ca2+]i were higher in controls during the proliferative phase, while adenomyosis patients showed elevated ESR1 and [Ca2+]i in both proliferative and secretory phases; oestradiol rapidly increased [Ca2+]i in JZSMCs from both groups. Pretreatment with the ESR1 antagonist ICI 182,780 reduced the oestradiol-induced increase in [Ca2+]i in both groups (though the between-group differences were not significant), while extracellular calcium removal did not change the effect; phospholipase C inhibition (U73122) and a calcium signaling modulator (2-aminoethoxydiphenyl borate) significantly reduced the oestradiol-induced calcium flux, and thapsigargin-depleted cells showed no oestradiol-driven flux, indicating ESR1-mediated, PLC-dependent calcium flux. A stated limitation is the small, tissue-isolation-based sample with membrane/protein and calcium-flux assays rather than direct functional peristalsis measurements. Relevance to endometriosis: although the paper focuses on adenomyosis, it discusses adenomyosis-related uterine junctional zone biology and ESR1 and calcium dysregulation in a disease context adjacent to endometriosis.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Calcium Estradiol Estrogen Receptor alpha Menstrual Cycle Myometrium Uterus Adenomyosis Calcium Cells, Cultured Estradiol Estradiol Estrogen Receptor alpha Female Humans Menstrual Cycle Myometrium Myometrium Uterus Uterus

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