Association of protease activity against IGFBP-3 with peritoneal fluid mitogens: possible implications for the ectopic growth of endometrial cells in women with endometriosis.
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Peritoneal fluid fractions with mitogenic and IGFBP-3-cleaving activity containing proteins between 50-25 kD were identified in women with and without endometriosis.
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Abstract
BACKGROUND: Recent data indicate the implication of an IGF/IGFBP/protease system in the development of ectopic implants of endometrium (endometriosis) in the pelvic cavity. MATERIAL AND METHODS: We characterized mitogens and protease(s) against IGFBP-3 in the peritoneal fluid of women with or without endometriosis using preparative isoelectric focusing (IEC) Proteineous fractions were tested for a) mitogenic activity on NIH/3T3 cells and KLE endometrial-like cells, b) proteinolytic activity against radiolabeled IGFBP-3, and c) protein analysis using SDS-PAGE. RESULTS: A significant increase in the DNA content of cultured cell lines was found in PF fractions having slightly acidic or alkaline pH and containing mainly proteins with molecular weights between 50 and 25 kD. These fractions were also found to cleave IGFBP-3 into 25, 18 and 14 kD fragments. Both activities were present in the PF of subjects with or without endometriosis. CONCLUSION: The preparative isoelectric focusing system would be an advantageous approach to investigate the possible implication of the PF proteases with IGFBP-3 limited proteolysis generating PF mitogen(s) which possibly participate in the pathophysiology of endometriosis.
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Cited by (12)
- MicroRNA-210-3p Regulates Endometriotic Lesion Development by Targeting IGFBP3 in Baboons and Women with Endometriosis 2023
- Endometriosis 2013
- Insulinlike Growth Factor-1Ec (MGF) Expression in Eutopic and Ectopic Endometrium: Characterization of the MGF E-Peptide Actions In Vitro 2010
- A prospective study of body size during childhood and early adulthood and the incidence of endometriosis 2010
- Expression and role of peptides, proteins and growth factors in the pathogenesis of endometriosis 2009
- Endometriosis 2009
- Insulin‐Like Growth Factor–1 Isoform mRNA Expression in Women with Endometriosis 2006
- Glucocorticoid receptor function suppresses insulin-like growth factor 1 activity in human KLE endometrial-like cells. 2004
- Urokinase‐Type Plasminogen Activator and Insulin‐Like Growth Factor‐Binding Protein 3 mRNA Expression in Endometriotic Lesions and Eutopic Endometrium 2003
- Macrophages, Oxidation, and Endometriosis 2002
- Increased Insulin-like Growth Factor 1 Activity Can Rescue KLE Endometrial-like Cells from Apoptosis 2001
- Immunohistochemical localization of insulin-like growth factor–binding protein-3 in eutopic and ectopic endometrial tissues 1999
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