Current status and future trends of the clinical practice of human oocyte in vitro maturation

Robert B. Gilchrist, Johan Smitz, Jeremy G. Thompson
In: Human Assisted Reproductive Technology · 2011 · pp. 186–198 · doi:10.1017/cbo9780511734755.018 · W2504833838
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This review discusses non-invasive proteomic approaches, particularly mass spectrometry, for analyzing proteins in biological fluids related to human oocyte in vitro maturation.

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This chapter evaluates the efficacy, safety, and clinical barriers of human oocyte in vitro maturation as an alternative to conventional in vitro fertilization. The authors note that while IVM eliminates the need for high-dose follicle-stimulating hormone stimulation, current protocols result in compromised embryo development rates, lower implantation potential, and increased early miscarriage risks. Despite these efficiency drawbacks, the text concludes that IVM carries no greater risk of adverse outcomes for mothers or children compared to standard IVF procedures. Relevance to endometriosis: listed as one indication for GnRH antagonists, though the paper's main focus is uterine fibroids.

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Abstract

This chapter focuses on the non-invasive approaches which largely concentrate on proteomics analyses. Proteins are generated from the genome with signal sequences that direct them to the cell membrane or are secreted in the extracellular environment. Secreted proteins are often post-translationally modified, in particular by glycosylation. These proteins are then released into the interstitial environment where they may enter body fluids such as the blood system and, in the case of endometriosis, be detected in endometrial, peritoneal, or follicular fluids. In most proteomic approaches, mass spectrometry (MS) plays a part in the analyses. Electrospray ionization (ESI) is the most common method for sample introduction into the MS. Matrix assisted laser desorption ionization (MALDI) is also be used as an alternative to ESI. Two-dimensional gel analyses have traditionally been used for the differential analysis of proteomes and differences may be observed and selected visually or using image analysis of scanned gels.
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Human Assisted Reproductive Technology Buy print or eBook [Opens in a new window] Future Trends in Laboratory and Clinical Practice - Human Assisted Reproductive Technology - Human Assisted Reproductive Technology - Copyright page - Dedication - Contents - Contributors - Preface - Acknowledgments - Chapter 1 Use of robotics in reproductive surgery - Chapter 2 Removal of hydrosalpinges and uterine leiomyoma to improve IVF outcome - Chapter 3 Ovarian endometriomas: - Chapter 4 Impact of uterine cavity abnormalities on IVF and pretreatment cavity evaluation - Chapter 5 Sperm retrieval techniques - Chapter 6 New concepts in ovarian stimulation - Chapter 7 GnRH antagonists in ART - Chapter 8 Pharmacogenetics of ovarian stimulation in the twenty-first century - Chapter 9 Non-invasive diagnosis of endometriosis with proteomic technologies - Chapter 10 Antiangiogenic agents for endometriosis - Chapter 11 New concepts in the management of polycystic ovary syndrome - Chapter 12 Adjuvant therapy to increase implantation rates - Chapter 13 Ovarian hyperstimulation syndrome - Chapter 14 The evidence-based practice of assisted reproduction - Chapter 15 Three-dimensional in vitro ovarian follicle culture - Chapter 16 Artificial gametes - Chapter 17 Current status and future trends of the clinical practice of human oocyte in vitro maturation - Chapter 18 Oocyte and zona imaging - Chapter 19 Granulosa cells and their impact on human ART - Chapter 20 Sperm diagnosis: - Chapter 21 Embryo culture in the twenty-first century - Chapter 22 Morphometric analyses of embryos - Chapter 23 Embryo biopsy: - Chapter 24 Analysis of the embryonic transcriptome - Chapter 25 Analysis of embryo-derived factors as markers of developmental potential and viability - Chapter 26 Proteomics analysis of the endometrium and embryo. Can we improve IVF outcome? - Chapter 27 Analysis of embryo metabolism and the metabolome to identify the most viable embryo within a cohort - Chapter 28 Oocyte and embryo cryopreservation - Index Published online by Cambridge University Press: 16 May 2011 Book contents - Human Assisted Reproductive Technology - Human Assisted Reproductive Technology - Copyright page - Dedication - Contents - Contributors - Preface - Acknowledgments - Chapter 1 Use of robotics in reproductive surgery - Chapter 2 Removal of hydrosalpinges and uterine leiomyoma to improve IVF outcome - Chapter 3 Ovarian endometriomas: - Chapter 4 Impact of uterine cavity abnormalities on IVF and pretreatment cavity evaluation - Chapter 5 Sperm retrieval techniques - Chapter 6 New concepts in ovarian stimulation - Chapter 7 GnRH antagonists in ART - Chapter 8 Pharmacogenetics of ovarian stimulation in the twenty-first century - Chapter 9 Non-invasive diagnosis of endometriosis with proteomic technologies - Chapter 10 Antiangiogenic agents for endometriosis - Chapter 11 New concepts in the management of polycystic ovary syndrome - Chapter 12 Adjuvant therapy to increase implantation rates - Chapter 13 Ovarian hyperstimulation syndrome - Chapter 14 The evidence-based practice of assisted reproduction - Chapter 15 Three-dimensional in vitro ovarian follicle culture - Chapter 16 Artificial gametes - Chapter 17 Current status and future trends of the clinical practice of human oocyte in vitro maturation - Chapter 18 Oocyte and zona imaging - Chapter 19 Granulosa cells and their impact on human ART - Chapter 20 Sperm diagnosis: - Chapter 21 Embryo culture in the twenty-first century - Chapter 22 Morphometric analyses of embryos - Chapter 23 Embryo biopsy: - Chapter 24 Analysis of the embryonic transcriptome - Chapter 25 Analysis of embryo-derived factors as markers of developmental potential and viability - Chapter 26 Proteomics analysis of the endometrium and embryo. Can we improve IVF outcome? - Chapter 27 Analysis of embryo metabolism and the metabolome to identify the most viable embryo within a cohort - Chapter 28 Oocyte and embryo cryopreservation - Index This chapter examines the process of in vitro maturation (IVM), its efficacy and safety, and explores why it is not more routinely used to treat infertility and what is required to make it a more viable clinical option. The treatment of infertility is based on in vitro fertilization (IVF) as the technology of choice. IVM is an alternative approach for generating mature oocytes that eliminates or significantly reduces the need for hormonal stimulation of the ovary. The benefit of IVM is that the procedure removes the need to administer large multiple doses of follicle stimulating hormone (FSH) normally used in conventional IVF treatment to women. Current IVM conditions compromise subsequent embryo development rates following fertilization. It is concluded that IVM, despite the reduced efficiencies of implantation potential and increased early miscarriage, should be viewed as having no risk of an adverse outcome to mother and child compared with conventional IVF. - Type - Chapter - Information - Human Assisted Reproductive TechnologyFuture Trends in Laboratory and Clinical Practice, pp. 186 - 198Publisher: Cambridge University PressPrint publication year: 2011 Accessibility compliance for the HTML of this chapter is currently unknown and may be updated in the future. - 11 - Cited by To save this book to your Kindle, first ensure [email protected] is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about saving to your Kindle. Note you can select to save to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be saved to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply. Find out more about the Kindle Personal Document Service. - Current status and future trends of the clinical practice of human oocyte in vitro maturation - - Book: Human Assisted Reproductive Technology - Online publication: 16 May 2011 To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Dropbox. - Current status and future trends of the clinical practice of human oocyte in vitro maturation - - Book: Human Assisted Reproductive Technology - Online publication: 16 May 2011 To save content items to your account, please confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your account. Find out more about saving content to Google Drive. - Current status and future trends of the clinical practice of human oocyte in vitro maturation - - Book: Human Assisted Reproductive Technology - Online publication: 16 May 2011

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endometriosis

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