Establishment and Characterization of a Stromal Cell Line Derived From a Patient With Thoracic Endometriosis

Gogusev J, Jean Gogusev, J Gogusev, Yves Lepelletier, Y Lepelletier, L El Khattabi, Khattabi LE, Laïla El Khattabi, Mădălina Grigoroiu, M Grigoroiu, Pierre Validire, P Validire, Validire P
Reproductive sciences (Thousand Oaks, Calif.) · 2019 · vol. 27(8) , pp. 193371911983347 · doi:10.1177/1933719119833475 · PMID:30819050 · W2918674052
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Researchers established and characterized a stromal cell line derived from a patient with thoracic endometriosis to investigate the disease's biological mechanisms.

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Abstract

Thoracic endometriosis (TE) syndrome is a clinical condition known as an extrapelvic form of endometriosis with the presence of functioning endometrial tissue involving lung parenchyma, pleura, chest wall, or diaphragm. In an effort to obtain an endometriosis ex vivo model, we established the spontaneously growing TH-EM1 cell line from endometriotic implants in lung parenchyma from a woman with TE. Maintained in long-term culture, the cells grew as large mesenchymal-like cells with a doubling time between 5 and 6 days. Treatment with medroxyprogesterone acetate (10-7 mol/L) inhibited the TH-EM1 cells growth and induced morphological changes to an epithelial-like cells. Strong expression of the nuclear estrogen receptors, progesterone receptors, and erytropoietin receptors were found in both the pulmonary implant and the TH-EM1 cells by immunohistochemical analysis. Consistent immunoreactivity of TH-EM1 cells for CD9, CD13, CD73, CD90, CD105, and CD157 was revealed by flow cytometry. Likewise, the embryonic markers, SRY-box 2 (SOX-2) and the Nanog molecules, were detected in 76% and 52% of the cells, while fetal hemoglobin and α-globin were detected in 76% and 65% of TH-EM1 cells, respectively. By RHG banding, normal metaphases were observed, while the microarray chromosomal analysis showed gains of DNA sequences located on the segments 8p23.1, 11p15.5, and 12p11.23. The described in vitro cellular model can serve as a useful tool to study the pathogenesis of endometriosis and to improve the knowledge of molecular mechanisms controlling the endometriotic cell dissemination potential.

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endometriosisthoracic_endometriosis

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