Two-way communication between endometrial stromal cells and monocytes
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This study demonstrated bidirectional communication between human endometrial stromal cells and U937 monocytes, evidenced by changes in gene expression upon treatment with conditioned media from the other cell type and hormonal treatments.
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The study examined bidirectional communication between cultured human monocytes and endometrial stromal cells and assessed stromal cell gene-expression responses to estradiol alone or combined with medroxyprogesterone acetate, using a telomerase-immortalized human endometrial stromal cell line (T-HESC) and the U937 monocyte line. Cells were exposed to estradiol ± medroxyprogesterone acetate ± monocyte-conditioned medium (for T-HESC) or to U937-conditioned medium via T-HESC-conditioned medium (for monocytes), and gene expression changes were measured using DNA microarrays. The authors found that gene expression was altered in both cell types, demonstrating two-way communication between U937 monocytes and T-HESC. A major limitation is that the experiments used immortalized cell lines rather than primary patient-derived cells. Relevance to endometriosis: the paper is included in the corpus because it explicitly investigates immune cell–endometrial cell signaling relevant to inflammatory mechanisms discussed in endometriosis research, though endometriosis itself is not experimentally modeled beyond contextual literature.
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Abstract
Immune system cells and cells of the endometrium have long been proposed to interact in both physiological and pathological processes. The current study was undertaken to examine communication between cultured monocytes and endometrial stromal cells and also to assess responses of endometrial stromal cells for treatment with estradiol (E) in the absence and presence of medroxyprogesterone acetate (P). A telomerase-immortalized human endometrial stromal cell (T-HESC) line and the U937 monocyte cell line were used. Telomerase-immortalized human endometrial stromal cells were treated with E +/- P +/- monocyte conditioned medium; U937 were treated +/- T-HESC conditioned medium. Gene expression in response to treatment was examined by DNA microarray. Bidirectional communication, as demonstrated by changes in gene expression, clearly occurred between U937 monocytes and T-HESC.
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Abstract
Immune system cells and cells of the endometrium have long been proposed to interact in both physiological and pathological processes. The current study was undertaken to examine communication between cultured monocytes and endometrial stromal cells and also to assess responses of endometrial stromal cells for treatment with estradiol (E) in the absence and presence of medroxyprogesterone acetate (P). A telomerase-immortalized human endometrial stromal cell (T-HESC) line and the U937 monocyte cell line were used. Telomerase-immortalized human endometrial stromal cells were treated with E ± P ± monocyte conditioned medium; U937 were treated ± T-HESC conditioned medium. Gene expression in response to treatment was examined by DNA microarray. Bidirectional communication, as demonstrated by changes in gene expression, clearly occurred between U937 monocytes and T-HESC.
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Klinkova, O., Hansen, K.A., Winterton, E. et al. Two-Way Communication Between Endometrial Stromal Cells and Monocytes. Reprod. Sci. 17, 125–136 (2010). https://doi.org/10.1177/1933719109348922
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DOI: https://doi.org/10.1177/1933719109348922
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