PENGARUH EKSTRAK DAUN KELOR (Moringa oleiferaLam.) \nTERHADAP EKSPRESI AROMATASE P450 DAN \nAPOPTOSIS SEL GRANULOSA PADA MENCIT \n(Mus musculus) MODEL ENDOMETRIOSIS

YUSRI DWI LESTARI, 011524653008
2018 · W7033027163
Lab / animal OA: green CC0
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This thesis investigated the effects of Moringa oleifera leaf ethanol extract on P450 aromatase expression and granulosa cell apoptosis in a murine model of endometriosis. The study utilized twenty-four female mice, inducing endometriosis via cyclosporin and human endometrial serum, then administering the extract to the treatment group over fourteen days while maintaining a placebo control. Results indicated that the treated mice exhibited significantly lower levels of both aromatase P450 expression and apoptotic granulosa cells compared to the untreated control group. This paper is centrally about endometriosis — specifically examining how Moringa oleifera extract modulates inflammatory and apoptotic pathways within an established experimental disease model.

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Abstract

Endometriosis merupakan suatu kondisi inflamasi kronis dan terdapat \njaringan endometrium yang tumbuh diluar kavum uteri. Jaringan endometrium ini \nberkaitan dengan nyeri kronis, radang panggul, dan infertilitasyang sering diderita \nperempuan usia reproduksi. Aromatase berperan dalam meningkatkan radang \nmemalui meningkatkan estrogen. Apoptosis sel granulosa yang meningkat pada \nendometriosis menyebabkan infertilitas. Daun kelor mengandung flavonoid yang \ndapat menghambat Nuclear factor kappa β(Nfkβ) dan Cyclooxygenase-2 (COX- \n2). Penghambatan Nfkβmampu menghambat apoptosis melalui jalur Tumor \nNecrosis Factor-α (TNF-α). Penghambatan COX-2mampu menghambat \naromatase melalui jalur Prostaglandin E2 (PGE2). \nTujuan penelitian ini adalah membuktikan ekspresi Aromatase P450 dan \njumlah apoptosis sel granulosa pada mencit (Mus musculus) model endometriosis \nyang diberi ekstrak etanol daun kelor (Moringa oleifera Lam.) lebih rendah \ndibandingkan dengan kelompok yang tidak diberi. \nSubyek penelitian menggunakan mencit betina yang dibagi 2 kelompok \nterdiri dari 1 kelompok kontrol dan 1 kelompok perlakuan, setiap kelompok terdiri \ndari 12 hewan coba. Hari ke 14 pembuatan model endometriosis, sebagian mencit \ndikorbankan untuk pembuktian. Kelompok kontrol diberi plasebo (Na-CMC) dan \nkelompok perlakuan diberi ekstrak etanol daun kelor selama 14 hari dengan dosis \n0,35 mg/gBB. Hari ke 29 dilakukan pembedahan untuk mengambil sampel \njaringan endometriosis dan ovarium untuk dilakukan pemeriksaan \nimunohistokimia ekspresi aromatase P450 dan apoptosis sel granulosa. \nHasil uji statistik menunjukkan bahwa terdapat perbedaan bermakna \ndengan nilai p=0,000<0,005 pada ekspresi aromatase P450 dan terdapat \nperbedaan bermakna dengan nilai p=0,001<0,05 pada jumlah apoptosis sel \ngranulosa.Kesimpulannya adalah Ekspresi Aromatase P450 dan jumlah apoptosis \nsel granulosa pada mencit (Mus musculus) model endometriosis yang diberi \nekstrak etanol daun kelor (Moringa oleifera Lam.) lebih rendah dibandingkan \ndengan kelompok yang tidak diberi.
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Abstract

THE EFFECT OF MORINGA OLEIFERA LEAFEXTRACTIONTO P450 AROMATASE EXPRESSION AND GRANULOSA CELL APOPTOSIS IN ENDOMETRIOSIS MICE MODEL YusriDwi Lestari, HendyHendarto, Widjiati Endometriosis is a chronic inflammation condition with the presence of endometrial tissue outside of cavum uteri. This endometrial tissue is related to the chronic pain, pelvic inflammatory disease, as well as infertility mostly in reproductive age d woman. The primary function of aromatase is to induce the inflammation through the estrogen upregulation. The increase of granulosa cell apoptosis in endometriosis could cause the infertility. The Moringa oleifera leaf has a flavonoid compound that affec ts the attenuation of Nuclear factor kappa β(Nfkβ) and Cyclooxygenase-2 (COX-2). The limitation of Nfkβ could prevent the apoptotic event through Tumor Necrosis Factor -α (TNF-α) pathway. In addition, the COX-2 limitation could reduce aromatase through Pro staglandin E2 (PGE2) pathway. The aims of this study were to prove that P450 aromatase expression and the number of apoptotic granulosa cells in endometriosis mice model that were treated with ethanol extraction from Moringa oleifera leaf was lower than th e untreated group. The subject of this study were 24 female mice ( Mus musculus) comprised to 1 control group and 1 treated group, with each group contains 12 mice. The endometriosis mice model were generated in the first 14 days by the injection of cyclosporin, extract serum of the human’s endometriosis uterus, and estrogen.The control group was treated with placebo (Na -CMC) while the treatment group was treated with ethanol extraction from Moringa oleifera leaf for 14 consecutive days with dose dependent manner 0. 35 mg/g BW/ day. On the 29 th day, mice were sacrificed to derive the endometriosis tissue as well as the ovarium to assess the P450 aromatase expression and granulosa cell apoptosis. Statistical analysis showed there was a significant difference in aromatase P450 expression between two groups with P=0.000<0.05 as well as in the number of apoptotic granulosa cells with p =0.001<0.05. To summarize, the expression of aromatase P450 and granulosa cell apoptosis in endometriosis mice model with the administration by ethanol extraction from Moringa oleifera leaf are lower if compare with the untreated group.

Keywords

Moringa oleifera, aromatase P450, granulosa cells, apoptosis

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endometriosis

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