{"paper_id":"7cced330-e121-4f60-9ce1-39fe883dc002","body_text":"IR-PERPUSTAKAN UNIVERSITAS AIRLANGGA \nxiv \nTESIS PENGARUH EKSTRAK DAUN.. YUSRI DWI  \n \nABSTRAK \nPENGARUH EKSTRAK DAUN KELOR (Moringa oleifera Lam.) \nTERHADAP EKSPRESI AROMATASE P450  DAN APOPTOSIS  \nSEL GRANULOSA PADA MENCIT (Mus musculus)  \nMODEL ENDOMETRIOSIS \n \nYusri Dwi Lestari, HendyHendarto, Widjiati \n \nEndometriosis merupakan suatu kondisi inflamasi kronis dan terdapat \njaringan endometrium yang  tumbuh diluar kavum uteri. Jaringan endometrium ini \nberkaitan dengan nyeri kronis, radang panggul, dan infertilitas yang sering diderita \nperempuan usia reproduksi . Aromat ase berperan dalam meningkatkan radang \nmemalui meningkatkan estrogen. Apoptosis sel granulosa yang meningkat pada \nendometriosis menyebabkan infertilitas.  Daun kelor mengandung flavonoid yang \ndapat menghambat Nuclear factor kappa β (Nfkβ) dan Cyclooxygenase-2 (COX-\n2). Penghambatan Nfk βmampu menghambat a poptosis melalui jalur Tumor \nNecrosis Factor -α (TNF-α). Penghambatan COX -2mampu menghambat \naromatase melalui jalur Prostaglandin E2 (PGE2). \nTujuan penelitian ini adalah membuktikan ekspresi Aromatase P450  dan \njumlah apoptosis sel granulosa pada mencit ( Mus musculus) model endometriosis \nyang diberi ekstrak etanol daun kelor ( Moringa oleifera Lam.) lebih rendah \ndibandingkan dengan kelompok yang tidak diberi. \nSubyek penelitian menggunakan mencit betina yang dibagi 2 kelompok \nterdiri dari 1 kelompok kontrol dan 1 kelompok perlakuan, setiap kelompok terdiri \ndari 12 hewan coba. Hari ke 14 pembuatan model endometriosis, sebagian mencit \ndikorbankan untuk pembuktian. Kelompok kontrol diberi pla sebo (Na-CMC) dan \nkelompok perlakuan diberi ekstrak etanol daun kelor selama 14 hari dengan dosis \n0,35 mg/gBB. Hari ke 29 dilakukan pembedahan untuk mengambil sampel \njaringan endometriosis dan ovarium untuk dilakukan pemeriksaan \nimunohistokimia ekspresi aromatase P450 dan apoptosis sel granulosa. \nHasil u ji statistik me nunjukkan bahwa terdapat p erbedaan bermakna \ndengan nilai p =0,000<0,005 pada ekspresi aromatase P450 dan terdapat \nperbedaan bermakna dengan nilai p =0,001<0,05 pada jumlah apoptosis sel \ngranulosa.Kesimpulannya adalah Ekspresi Aromatase P450  dan jumlah apoptosis \nsel granulosa pada mencit ( Mus musculus ) model endometriosis yang diberi \nekstrak etanol daun kelor ( Moringa oleifera Lam.) lebih rendah dibandingkan \ndengan kelompok yang tidak diberi. \n \nKata kunci : daun kelor, aromatase P450, sel granulose, apoptosis \n \n \n \n\nIR-PERPUSTAKAN UNIVERSITAS AIRLANGGA \nxv \nTESIS PENGARUH EKSTRAK DAUN.. YUSRI DWI  \n \nABSTRACT \n \nTHE EFFECT OF MORINGA OLEIFERA LEAFEXTRACTIONTO P450 \nAROMATASE EXPRESSION AND GRANULOSA CELL APOPTOSIS  \nIN ENDOMETRIOSIS MICE MODEL \n \nYusriDwi Lestari, HendyHendarto, Widjiati \n \nEndometriosis is a chronic inflammation condition with the presence of \nendometrial tissue outside of cavum uteri. This endometrial tissue is related to the \nchronic pain, pelvic inflammatory disease, as well as infertility mostly in \nreproductive age d woman. The primary function of aromatase is to induce the \ninflammation through the estrogen upregulation. The increase of granulosa cell \napoptosis in endometriosis could cause the infertility. The Moringa oleifera leaf \nhas a flavonoid compound that affec ts the attenuation of Nuclear factor kappa \nβ(Nfkβ) and Cyclooxygenase-2 (COX-2). The limitation of Nfkβ could prevent \nthe apoptotic event through Tumor Necrosis Factor -α (TNF-α) pathway. In \naddition, the COX-2 limitation could reduce aromatase through  Pro staglandin E2 \n(PGE2) pathway. \nThe aims of this study were to prove that P450 aromatase expression and \nthe number of apoptotic granulosa cells in endometriosis mice model that were \ntreated with ethanol extraction from Moringa oleifera  leaf was lower than th e \nuntreated group. \nThe subject of this study were 24 female mice ( Mus musculus) comprised \nto 1 control group and 1 treated group, with each group contains 12 mice. The \nendometriosis mice model were generated in the first 14 days  by the injection of \ncyclosporin, extract serum of the human’s endometriosis uterus, and estrogen.The \ncontrol group was treated with placebo (Na -CMC) while the treatment group was \ntreated with ethanol extraction from Moringa oleifera leaf for 14 consecutive days \nwith dose dependent manner 0. 35 mg/g BW/ day. On the 29 th day, mice were \nsacrificed to derive the endometriosis tissue as well as the ovarium to assess the \nP450 aromatase expression and granulosa cell apoptosis. \nStatistical analysis showed there was a significant difference in  aromatase \nP450 expression between two groups with P=0.000<0.05 as well as in the number \nof apoptotic granulosa cells with p =0.001<0.05. To summarize, the expression of \naromatase P450 and granulosa cell apoptosis in endometriosis mice model with \nthe administration by ethanol extraction from Moringa oleifera leaf are lower if \ncompare with the untreated group. \n \nKeywords: Moringa oleifera, aromatase P450, granulosa cells, apoptosis","source_license":"CC0","license_restricted":false}