Effect of steroid biosynthesis modifiers on progesterone biotransformation by recombinant yeasts expressing cytochrome P450cl7

V. M. Shkumatov; Elena Usova; N. S. Frolova; Gerold Barth; Stephan Mauersberger

doi:10.1134/s1990750807010131

Effect of steroid biosynthesis modifiers on progesterone biotransformation by recombinant yeasts expressing cytochrome P450cl7

V. M. Shkumatov, Elena Usova, N. S. Frolova, Gerold Barth, Stephan Mauersberger

In: Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry · 2007 · vol. 1(1) , pp. 87–94 · doi:10.1134/s1990750807010131 · W2022707899

article OA: closed CC0

View on OpenAlex View at publisher

⚙ AI-generated summary by qwen3.7-flash, 2026-08-16 ⓘ

This study evaluated steroid biosynthesis modifiers on progesterone biotransformation by recombinant yeasts expressing cytochrome P450cl7, identifying ketokonazole and mifepriston as low-affinity competitive inhibitors and ranking their effects on catalytic parameters.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

Abstract

Using recombinant microorganisms S. cerevisiae GRF18/YEp 5117α, expressing bovine adrenocortical cytochrome P450cl7, we have studied the effect of various modifiers of steroid biosynthesis on the relationship between reactions of the 17α-hydroxylation and 20α-reduction of progesterone. Dexamethasone and metyrapone had no effect on the reaction of progesterone 17α-hydroxylation and 20α-reduction of 17α-hydroxyprogesterone. Mifepriston and danazol did not covalently modify amino acid residues of the cytochrome P450cl7 or its heme group under the conditions of progesterone biotransformation by recombinant yeasts. Ketokonazole, mifepriston and danazol were found to be low-affinity competitive inhibitors, but the 20-dihydroderivatives of progesterone were mixed type inhibitors of the cytochrome P450cl7. All modifiers used did not affect the functional properties of the yeast analog of 20α-hydroxysteroid dehydrogenase. Based on the effect on catalytic parameters of the cytochrome P450cl7, the all modifiers used can be arranged in the following order: 20β-dihydroprogesterone (maximal effect) > mifepriston = ketokonazole > 20α-dihydroprogesterone > danazol > dexamethasone, metyrapone (without effect).

My notes (saved in your browser only)

Citation neighborhood (sparse)

Too few in-corpus citations on either side for a chart; here are the lists.

Cites (1)

Microbial Transformation of Danazol 2002

References (26)

Microbial Transformation of Danazol via openalex
doi:10.1023/a:1015546211706 via openalex
doi:10.1074/jbc.m105438200 via openalex
doi:10.1016/0022-4731(87)90147-6 via openalex
doi:10.1016/0960-0760(94)90206-2 via openalex
doi:10.1038/nbt775 via openalex
doi:10.1016/s0021-9258(19)69538-4 via openalex
doi:10.1042/bj3300967 via openalex
doi:10.1074/jbc.m006655200 via openalex
doi:10.1097/00008571-199111000-00005 via openalex
doi:10.1016/s0021-9258(17)43191-7 via openalex
doi:10.1023/a:1015290108071 via openalex
doi:10.1016/0167-4838(84)90350-9 via openalex
doi:10.1038/nbt0298-186 via openalex
doi:10.1002/yea.882 via openalex
doi:10.1074/jbc.m100040200 via openalex
doi:10.1016/s0960-0760(00)00137-0 via openalex
doi:10.1016/s0960-0760(01)00172-8 via openalex
doi:10.1016/s0022-3565(24)38021-8 via openalex
doi:10.1089/dna.1990.9.27 via openalex
doi:10.1016/s0960-0760(03)00070-0 via openalex
doi:10.1021/bi002305w via openalex
doi:10.1016/0167-4838(84)90104-3 via openalex
doi:10.1124/dmd.30.9.985 via openalex
doi:10.1177/153537020222701104 via openalex
doi:10.1016/s0003-9861(02)00491-5 via openalex

Source provenance

openalex: last seen: 2026-05-10T10:26:51.820283+00:00
unpaywall: last seen: 2026-09-21T07:16:15.697306+00:00

License: CC0 · commercial use OK

[1] Microbial Transformation of Danazol 2002