miR-9-5p promotes the invasion and migration of endometrial stromal cells in endometriosis patients through the SIRT1/NF-κB pathway.

Jinyan Zheng, Shanshan Shao, Chanjuan Dai, Shan Guan, Hong Chen
International journal of clinical and experimental pathology · 2020 · vol. 13(7) , pp. 1859–1866 · PMID:32782715 · PMC7414472 · W3049722949
article OA: green CC0 ⤵ 2 in-corpus citations
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⚙ AI-generated summary by claude@2026-06, 2026-06-07 ⓘ

miR-9-5p, highly expressed in endometriosis, promotes endometrial stromal cell invasion and migration by inhibiting SIRT1 and activating the NF-κB pathway.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-07 ⓘ

This study investigated miR-9-5p expression and its effects on invasion and migration of endometrial stromal cells using biopsies from 17 eutopic, 19 ectopic, and 13 normal endometrium patients, with RT-qPCR, western blot, dual luciferase reporter assays, and in vitro migration (scratch) and invasion (Transwell) tests. The authors found miR-9-5p was elevated and SIRT1 was reduced in endometriosis tissues, with miR-9-5p negatively correlated with SIRT1 mRNA, and that miR-9-5p directly targeted SIRT1 expression. Overexpression of miR-9-5p (mimics) increased migration distance and Transwell invasion, while miR-9-5p inhibition decreased these behaviors, alongside corresponding changes in nuclear p65 phosphorylation consistent with activation of the NF-κB pathway; they used resveratrol/EX527 to further modulate SIRT1 and align NF-κB signaling outcomes. The paper does not explicitly discuss limitations such as sample size, in vivo validation, or the extent to which cell line-free primary culture conditions replicate disease biology, but it relies on in vitro assays of endometrial stromal cells. This paper is centrally about endometriosis—specifically, how miR-9-5p promotes endometrial stromal cell invasion and migration via the SIRT1/NF-κB pathway.

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Abstract

OBJECTIVE: The present study was designed to investigate the expression of miR-9-5p and to study the effect of miR-9-5p expression on the invasion and migration of endometrial stromal cells in endometriosis patients. METHODS: We recruited 17 eutopic endometrium patients, 19 ectopic endometrium patients, and 13 normal endometrium patients, and we measured their miR-9-5p and SIRT1 expressions. Western blot was used to measure the protein expressions, and cellular immunofluorescence was used to check the positions of the p65 position protein in cells. A Transwell chamber and cell scratch tests were used to test cell invasion and migration, respectively. RESULTS: miR-9-5p was highly expressed, and SIRT1 was lowly expressed in the endometria of the endometriosis patients, and there was a negative correlation between miR-9-5p and SIRT1 mRNA in the endometriosis patients. A dual luciferase reporter gene system showed that miR-9-5p targeted the inhibition of SIRT1 expression in the endometrial stromal cells. Moreover, the up-regulation of miR-9-5p expression using the miR-9-5p-mimics significantly increased the distance of endometrial stromal cell migration and the number of cells that entered into the lower chamber of the Transwell chamber, and the down-regulation of miR-9-5p using the miR-9-5p-inhibitor significantly decreased the distance of endometrial stromal cell migration and the number of cells that entered into the lower chamber of the Transwell chamber. Moreover, the miR-9-5p-mimics significantly increased the expressions of the P-p65/p65 protein and the 65 protein in the nuclei, and the miR-9-5p-inhibitor significantly decreased the expressions of the P-p65/p65 protein and the 65 protein in the nuclei. CONCLUSION: miR-9-5p is highly expressed in the endometria of endometriosis patients, and miR-9-5p can promote the invasion and migration of endometrial stromal cells in vitro by targeting the SIRT1 expression via the NF-κB pathway.

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endometriosis

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