Enhanced purification of uterine smooth muscle cells from adenomyosis using a novel dual-enzyme digestion method

Yishen Rong, Yichen Chen, Jue Zhu, Yuhui Sun, Qiming Wang, Jing Zhang
New biotechnology · 2024 · vol. 84 , pp. 151–161 · doi:10.1016/j.nbt.2024.11.001 · PMID:39521046 · W4404147242
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⚙ AI-generated summary by claude@2026-06, 2026-06-06 ⓘ

A novel dual-enzyme digestion method, DM4, yields higher purity and proliferation of mature uterine smooth muscle cells compared to conventional tissue adherence methods.

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Abstract

Uterine adenomyosis causing attention to the abnormal smooth muscle layer has recently received increasing attention, which has created the need for a method that can efficiently collect high purity uterine smooth muscle cells (USMC) in a laboratory setting. In this study, we explored the composition ratios of the digestion solution to obtain the optimal digestion solution (DM4). Furthermore, we tested the superiority of the two methods of obtaining adenomyotic uterine smooth muscle by comparing DM4 with the conventional tissue adhesion method by growth rate, single-cell RNA sequencing, and purity of fluorescence identification. The results demonstrated that USMCs produced by the DM4 digestion method exhibited significantly higher rates of proliferation and were more effective in generating mature smooth muscle cells of high purity compared to those obtained using tissue adherence methods, which is more inclined to isolate the progenitor cell population. This study presents a reliable method for isolating USMCs and provides a solid foundation for future studies on the etiology and mechanism of adenomyosis. • A novel dual-enzyme digestion method enhances USMC purity and enhances cell proliferation • The DM4 digestion yields high-purity mature USMCs, while tissue adhesion favors progenitor cells. • CCDC80 + cells undergo a transition from tissue adherence to become fibroblast-like cells that express COLIII.

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Adenomyosis Myocytes, Smooth Muscle Myocytes, Smooth Muscle Myocytes, Smooth Muscle Myocytes, Smooth Muscle Myocytes, Smooth Muscle

Citation neighborhood

Papers in the corpus that this work cites (lower rings, blue) and that cite this one (upper rings, green). Dot size scales with the paper's in-corpus citation count — bigger dot = more influential within the endo/adeno field. Click a dot to open that paper. [ expand to 2 hops ] — adds papers reached through this work's immediate citers/citees. Heavier; up to 60 extra dots.

References (33)

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