Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application

Thangapandi K; Thangapandi Kalyani; Arumugam Sangili; Amalesh Nanda; Nanda A; Prakash S; Sengodu Prakash; Ajeet Kaushik; Kumar Jana S; Saikat Kumar Jana

doi:10.1016/j.bioelechem.2021.107740

Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application

Thangapandi K, Thangapandi Kalyani, Arumugam Sangili, Amalesh Nanda, Nanda A, Prakash S, Sengodu Prakash, Ajeet Kaushik, Kumar Jana S, Saikat Kumar Jana

Bioelectrochemistry (Amsterdam, Netherlands) · 2021 · vol. 139 , pp. 107740 · doi:10.1016/j.bioelechem.2021.107740 · PMID:33524653 · W3118689635

article OA: closed CC0 ⤵ 11 in-corpus citations

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Researchers developed a bio-nanocomposite electrochemical immunosensor for label-free detection of endometriosis biomarkers.

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Abstract

In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application. Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics. Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1. Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy. To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA). The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA. We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.

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endometriosis

MeSH descriptors

Antigens, Tumor-Associated, Carbohydrate Biosensing Techniques Electrochemical Techniques Endometriosis Immunoassay Point-of-Care Testing Antigens, Tumor-Associated, Carbohydrate Biosensing Techniques Chitosan Chitosan Electrochemical Techniques Electrodes Endometriosis Female Gold Gold Graphite Graphite Humans Immunoassay

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Cited by (13)

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License: CC0 · commercial use OK

[1] Comparison of the clinical value of CA 19-9 versus CA 125 for the diagnosis of endometriosis via openalex

[2] Heavy black tea consumption and elevated CA 19-9 and CA 125 levels. A case report on a patient with ovarian endometriotic cysts via openalex

[3] Integrated Eutopic Endometrium and Non‐Depleted Serum Quantitative Proteomic Analysis Identifies Candidate Serological Markers of Endometriosis via openalex

[4] Label-Free Electrochemical Immunosensor Based on One-Step Electrochemical Deposition of AuNP-RGO Nanocomposites for Detection of Endometriosis Marker CA 125 via openalex

[5] Laparoscopic Management of Peritonitis Due to a Ruptured Ovarian Endometrioma with Extremely High Levels of Cancer Antigen–125 and Cancer Antigen–19-9 via openalex

[6] Metabolomics reveals perturbations in endometrium and serum of minimal and mild endometriosis via openalex

[7] doi:10.1016/j.electacta.2012.04.025 via openalex

[8] doi:10.1016/j.cocis.2006.08.001 via openalex

[9] doi:10.1016/j.cej.2015.08.158 via openalex

[10] doi:10.1016/j.snb.2014.03.011 via openalex

[11] doi:10.1016/j.aca.2016.08.015 via openalex

[12] doi:10.1016/j.bios.2015.09.051 via openalex

[13] doi:10.1016/j.bios.2014.10.066 via openalex

[14] doi:10.1021/ac034213t via openalex

[15] doi:10.1021/ac702203f via openalex

[16] doi:10.1021/acsomega.7b01029 via openalex

[17] W2463155735 via openalex

[18] doi:10.1016/j.bios.2018.09.016 via openalex

[19] doi:10.1016/j.bios.2018.06.010 via openalex

[20] doi:10.1016/j.snb.2018.03.178 via openalex

[21] doi:10.1016/j.foodchem.2018.10.024 via openalex

[22] doi:10.1016/j.chemosphere.2018.11.080 via openalex

[23] doi:10.1007/s00604-019-3319-7 via openalex

[24] doi:10.1038/s41598-018-33398-8 via openalex

[25] doi:10.1039/c8nr08741e via openalex

[26] doi:10.1016/j.jelechem.2018.10.058 via openalex

[27] doi:10.1007/s00604-019-3396-7 via openalex

[28] doi:10.1007/s00216-019-01979-9 via openalex

[29] doi:10.1016/j.bios.2019.111608 via openalex

[30] W6767911289 via openalex

[31] doi:10.1016/j.bios.2019.04.018 via openalex

[32] doi:10.1016/j.aca.2018.10.041 via openalex

[33] doi:10.1016/j.aca.2019.01.019 via openalex

[34] doi:10.1016/j.bios.2019.05.016 via openalex

[35] doi:10.1016/j.compositesb.2018.10.084 via openalex

[36] doi:10.1021/acsami.9b01617 via openalex

[37] doi:10.1016/j.matchemphys.2019.121877 via openalex

[38] doi:10.1016/s1452-3981(23)15953-0 via openalex

[39] doi:10.1021/jacs.9b03007 via openalex

[40] doi:10.1039/c7an00417f via openalex

[41] doi:10.1016/j.bios.2009.09.002 via openalex

[42] doi:10.1016/j.electacta.2018.09.108 via openalex

[43] doi:10.1016/j.measurement.2011.10.022 via openalex

[44] doi:10.1016/j.snb.2017.04.156 via openalex

[45] doi:10.1016/j.snb.2013.01.013 via openalex

[46] doi:10.1007/s00216-019-01867-2 via openalex

[47] doi:10.1021/ac049519u via openalex

[48] doi:10.1016/j.bios.2017.08.005 via openalex

[49] doi:10.1016/j.talanta.2014.02.032 via openalex

[50] doi:10.1016/j.bios.2015.08.047 via openalex

[51] doi:10.1021/acs.analchem.9b03855 via openalex

[52] doi:10.1016/j.bios.2017.04.020 via openalex

[53] doi:10.1016/s0302-4598(00)00129-x via openalex

[54] doi:10.1021/ja044764g via openalex

[55] doi:10.1016/j.snb.2018.01.092 via openalex

[56] doi:10.1021/ac303142e via openalex

[57] doi:10.1016/j.bios.2014.12.013 via openalex