Effects of aminophylline, imidazole and indomethacin on spontaneous and prostaglandin induced ovarian contractions in vitro.

Imidazole, acetylcholine, phenylephrine prostaglandin F2alpha, and methyl prostaglandin F2alpha increased the contractility of guinea pig and human ovaries in vitro. This effect was suppressed by aminophylline. Indomethacin inhibited ovarian contractions. The inhibitory effect of indomethacin was reversed by prostaglandin F2alpha or by its methyl derivative. Prostaglandin E2 decreased the amplitude and frequency of the spontaneous and prostaglandin F2alpha induced contractions of guinea pig ovaries in vitro. The study shows that compounds which interfere with cyclic AMP and prostaglandin metabolism affect ovarian contractility in vitro.