Decreased expression of human heat shock protein 70 in the endometria and pathological lesions of women with adenomyosis and uterine myoma after GnRH agonist therapy

Khaleque N Khan, Khan KN, Khaleque Newaz Khan, Michio Kitajima, Kitajima M, Hiraki K, Koichi Hiraki, Akira Fujishita, Fujishita A, Masahiro Nakashima, Nakashima M, Masuzaki H, Hideaki Masuzaki
European journal of obstetrics, gynecology, and reproductive biology · 2015 · vol. 187 , pp. 6–13 · doi:10.1016/j.ejogrb.2015.01.012 · PMID:25697974 · W1971634355
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⚙ AI-generated summary by gemini-2.5-flash-lite+body, 2026-06-12 ⓘ

GnRH agonist therapy significantly decreased heat shock protein 70 expression in women with adenomyosis and uterine myoma, suggesting reduced tissue stress reaction.

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⚙ AI-generated deep summary by claude@2026-06, 2026-06-12 · read from full text ⓘ

This case-controlled biological study assessed human heat shock protein 70 (HSP70) expression in eutopic endometria, autologous myometria, and pathological lesions from women undergoing surgery for adenomyosis (n=30) or uterine myoma (n=35), compared with controls (n=15), using immunohistochemistry and quantitative-histogram scoring; macrophage-associated inflammation was evaluated by CD68-positive Mϕ counts. HSP70 immunoexpression was significantly higher than in controls in endometria/myometria and lesions of both adenomyosis and myoma groups, and in adenomyosis endometrium HSP70 Q-H scores positively correlated with CD68-positive Mϕ numbers (r=0.388). In a subset treated with GnRH agonist for 3–6 months, HSP70 Q-H scores in lesions and endometria/myometria decreased compared with GnRH-untreated tissues. This paper is centrally about endometriosis and/or adenomyosis — it directly studies adenomyosis tissue stress/inflammation via HSP70 and its suppression after GnRH agonist therapy.

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Abstract

ObjectiveA prominent stress reaction in the pelvis of women with endometriosis and the role of human heat shock protein 70 (HSP70) in inflammation and the growth of endometriosis has been recently demonstrated. We report here expression of HSP70 in tissues derived from GnRH agonist (GnRHa)-untreated and -treated women with adenomyosis and uterine myoma.Study designThis is a case-controlled biological study. Biopsy specimens were collected from pathological lesions and eutopic endometria/autologous myometria of 30 women with adenomyosis, 35 women with uterine myoma and 15 control women during laparoscopy, laparotomy and hysteroscopy. Fourteen women with adenomyosis and 20 women with uterine myoma were treated with GnRHa for a variable period of 3-6 months before surgery. The immunoexpressions of HSP70 and CD68-positive Mϕ in endometria, lesions/myometria were examined by immunohistochemistry. The immunoreactivity of HSP70 in tissues was analyzed by quantitative-histogram (Q-H) scores.ResultsComparing to control women, HSP70 immunoexpression was significantly higher in endometria/myometria and pathological lesions of women with adenomyosis and myoma. A significant positive correlation between Q-H scores of HSP70 and CD68-positive Mϕ numbers was found in the endometria derived from women with adenomyosis (r=0.388). Treatment with GnRHa significantly decreased Q-H scores of HSP70 in pathological lesions and endometria/myometria of women with adenomyosis and uterine myoma comparing to similar tissues derived from GnRHa-untreated women.ConclusionA variable amount of tissue stress reaction occurred in endometria and pathological lesions of women adenomyosis and uterine myoma that can be effectively suppressed after GnRHa treatment.
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WEKO3 アイテム Decreased expression of human heat shock protein 70 in the endometria and pathological lesions of women with adenomyosis and uterine myoma after GnRH agonist therapy http://hdl.handle.net/10069/35116 http://hdl.handle.net/10069/35116d306f470-0482-4375-927a-b4fbae6a398c | 名前 / ファイル | ライセンス | アクション | |---|---|---| | EJOG187_6.pdf (745.7 kB) | | | アイテムタイプ | 学術雑誌論文 / Journal Article(1) | ||||||||||||||||| |---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---| | 公開日 | 2015-03-04 | ||||||||||||||||| | タイトル | |||||||||||||||||| | タイトル | Decreased expression of human heat shock protein 70 in the endometria and pathological lesions of women with adenomyosis and uterine myoma after GnRH agonist therapy | ||||||||||||||||| | 言語 | |||||||||||||||||| | 言語 | eng | ||||||||||||||||| | キーワード | |||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||| | 主題 | Adenomyosis | ||||||||||||||||| | キーワード | |||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||| | 主題 | Uterine myoma | ||||||||||||||||| | キーワード | |||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||| | 主題 | HSP70 | ||||||||||||||||| | キーワード | |||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||| | 主題 | Inflammation | ||||||||||||||||| | キーワード | |||||||||||||||||| | 主題Scheme | Other | ||||||||||||||||| | 主題 | GnRHa | ||||||||||||||||| | 資源タイプ | |||||||||||||||||| | 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | ||||||||||||||||| | 資源タイプ | journal article | ||||||||||||||||| | 著者 | Khan, Khaleque Newaz × Khan, Khaleque Newaz × Kitajima, Michio × Hiraki, Koichi × Fujishita, Akira × Nakashima, Masahiro × Masuzaki, Hideaki | ||||||||||||||||| | 抄録 | |||||||||||||||||| | 内容記述タイプ | Abstract | ||||||||||||||||| | 内容記述 | Objective A prominent stress reaction in the pelvis of women with endometriosis and the role of human heat shock protein 70 (HSP70) in inflammation and the growth of endometriosis has been recently demonstrated. We report here expression of HSP70 in tissues derived from GnRH agonist (GnRHa)-untreated and -treated women with adenomyosis and uterine myoma. Study design This is a case-controlled biological study. Biopsy specimens were collected from pathological lesions and eutopic endometria/autologous myometria of 30 women with adenomyosis, 35 women with uterine myoma and 15 control women during laparoscopy, laparotomy and hysteroscopy. Fourteen women with adenomyosis and 20 women with uterine myoma were treated with GnRHa for a variable period of 3?6 months before surgery. The immunoexpressions of HSP70 and CD68-positive M? in endometria, lesions/myometria were examined by immunohistochemistry. The immunoreactivity of HSP70 in tissues was analyzed by quantitative-histogram (Q-H) scores. Results Comparing to control women, HSP70 immunoexpression was significantly higher in endometria/myometria and pathological lesions of women with adenomyosis and myoma. A significant positive correlation between Q-H scores of HSP70 and CD68-positive M? numbers was found in the endometria derived from women with adenomyosis (r = 0.388). Treatment with GnRHa significantly decreased Q-H scores of HSP70 in pathological lesions and endometria/myometria of women with adenomyosis and uterine myoma comparing to similar tissues derived from GnRHa-untreated women. Conclusion A variable amount of tissue stress reaction occurred in endometria and pathological lesions of women adenomyosis and uterine myoma that can be effectively suppressed after GnRHa treatment. | ||||||||||||||||| | 書誌情報 | European Journal of Obstetrics & Gynecology and Reproductive Biology 巻 187, p. 6-13, 発行日 2015-04 | ||||||||||||||||| | 出版者 | |||||||||||||||||| | 出版者 | Elsevier Ireland Ltd. | ||||||||||||||||| | ISSN | |||||||||||||||||| | 収録物識別子タイプ | ISSN | ||||||||||||||||| | 収録物識別子 | 03012115 | ||||||||||||||||| | DOI | |||||||||||||||||| | 関連タイプ | isVersionOf | ||||||||||||||||| | 識別子タイプ | DOI | ||||||||||||||||| | 関連識別子 | 10.1016/j.ejogrb.2015.01.012 | ||||||||||||||||| | 権利 | |||||||||||||||||| | 権利情報 | c 2015 Elsevier Ireland Ltd. | ||||||||||||||||| | 権利 | |||||||||||||||||| | 権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in European Journal of Obstetrics & Gynecology and Reproductive Biology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Obstetrics & Gynecology and Reproductive Biology, 187, (2015) | ||||||||||||||||| | 著者版フラグ | |||||||||||||||||| | 出版タイプ | AM | ||||||||||||||||| | 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | ||||||||||||||||| | 引用 | |||||||||||||||||| | 内容記述タイプ | Other | ||||||||||||||||| | 内容記述 | European Journal of Obstetrics & Gynecology and Reproductive Biology, 187, pp.6-13; 2015 |

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Condition tags

adenomyosis

MeSH descriptors

Adenomyosis Adenomyosis Endometrium Gonadotropin-Releasing Hormone HSP70 Heat-Shock Proteins Leiomyoma Uterine Neoplasms Adenomyosis Adenomyosis Adult Case-Control Studies Endometrium Female Gonadotropin-Releasing Hormone Gonadotropin-Releasing Hormone HSP70 Heat-Shock Proteins Humans Immunohistochemistry Leiomyoma Leiomyoma

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