Production of various marker substances in human ovarian cancer cell lines of epithelial origin

Sekiya S, S Sekiya, N Tanaka, Tanaka N, H Takamizawa, Takamizawa H
Archives of gynecology and obstetrics · 1990 · doi:10.1007/BF02389582 · PMID:2078056
other OA: closed public-domain-us
Full text JSON View on PubMed View at publisher
⚙ AI-generated summary by gemini-2.5-flash-lite, 2026-06-13 ⓘ

This study examined 8 human ovarian cancer cell lines, finding significant heterogeneity in tumor marker production and observing progesterone or hCG production in serum-free conditions.

One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works

⚙ AI-generated deep summary by claude@2026-06, 2026-06-13 · read from full text ⓘ

The study examined tumor marker production and basic cellular characteristics in eight human ovarian cancer cell lines of epithelial origin, assessing karyotypes and the presence of specific markers in culture conditions with serum or synthetic serum-free media. Structural chromosomal abnormalities involving chromosomes 1, 3, and 6 were relatively common, cytoplasmic estradiol or progesterone receptors were not detected, and marker production (ferritin, tissue peptide antigen, carcinoembryonic antigen, CA125, and CA19-9) varied significantly between cell lines. Three lines were adapted to serum-free culture and, in small amounts, two of these produced progesterone or human chorionic gonadotropin, while the authors conclude that marker substances are not produced consistently by these ovarian epithelial cancer cells. Relevance to endometriosis: the paper does not explicitly discuss endometriosis or adenomyosis, and it was included in the corpus via a keyword match in the upstream search index.

Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works

Abstract

Some characteristics of cell biology and the production of various tumor markers were examined using 8 human ovarian cancer cell lines of epithelial origin. Structural abnormalities of chromosomes 1, 3, and 6 were relatively common karyotypic changes among the cell lines. Cytoplasmic estradiol or progesterone receptor was not detected in any of the cell lines. A significant heterogeneity of the production of various tumor markers (ferritin, tissue peptide antigen, carcinoembryonic antigen, carbohydrate antigens 125, and 19-9) was noted among the cell lines grown in culture medium supplemented with serum. Three of the 8 cell lines were adapted to proliferate in completely synthetic serum-free culture medium. In addition to marker substances described above, small amounts of progesterone or human chorionic gonadotropin were produced in 2 of the 3 cell lines grown in serum-free culture medium. These results indicate that various marker substances including tumor markers are not produced consistently by human ovarian cancer cells of epithelial origin.
Full text 3,748 characters · extracted from oa-doi-fallback · click to expand
Summary Some characteristics of cell biology and the production of various tumor markers were examined using 8 human ovarian cancer cell lines of epithelial origin. Structural abnormalities of chromosomes 1, 3, and 6 were relatively common karyotypic changes among the cell lines. Cytoplasmic estradiol or progesterone receptor was not detected in any of the cell lines. A significant heterogeneity of the production of various tumor markers (ferritin, tissue peptide antigen, carcinoembryonic antigen, carbohydrate antigens 125, and 19-9) was noted among the cell lines grown in culture medium supplemented with serum. Three of the 8 cell lines were adapted to proliferate in completely synthetic serum-free culture medium. In addition to marker substances described above, small amounts of progesterone or human chorionic gonadotropin were produced in 2 of the 3 cell lines grown in serum-free culture medium. These results indicate that various marker substances including tumor markers are not produced consistently by human ovarian cancer cells of epithelial origin. Similar content being viewed by others References Torosian MH (1988) The clinical usefulness and limitations of tumor markers. Surg Gynecol Obstet 166:567–579 Jacobs I, Bast RC jr (1989) The CA125 tumor-associated antigen: a review of the literature. Human Reproduct 4:1–12 Yamada T (1974) The cellular biology of a newly established cell line of human ovarian adenocarcinoma in vitro. Keio J Med 23:53–70 Kiyozuka Y, Imai S, Noda T, Morimoto J, Moriyama I, Ichijo M, Tsubura Y (1988) Establishment and characterization of human ovarian cystadenocarcinoma cell line (SHIN-3) which produce CA125 and TPA in vitro. J Jpn Soc Clin Cytol 27:926–935 Ishiwata I, Ishiwata C, Kobayashi N, Ishikawa H (1987) Characterization of newly established cell line HUOA from a human ovarian serous cystadenocarcinoma. Acta Obst Gynaec Jpn 39:831–836 Yamada T, Ueda M (1988) Establishment and characterization of a cell line (OMC-3) originating from human mucinous cystadenocarcinoma of the ovary. Human Cell 1:340–341 Fujii T (1989) Establishment and characterization of human ovarian endometrioid carcinoma cell line. Acta Obst Gynaec Jpn 41:161–166 Ishiwata I, Ishiwata C, Soma M, Ishikawa H (1986) Establishment and characterization of two human ovarian endometrioid carcinoma cell lines (with or without squamous cell component). Gynecol Oncol 25:95–107 Nishida M, Iwasaki H (1988) Establishment of a human ovarian clear cell carcinoma cell line (HAC-2 cells) in vitro. Human Cell 1:345–346 Ishiwata I, Ishiwata C, Soma M, Ishikawa H (1987) Establishment of HUOCA-II, a human ovarian clear cell adenocarcinoma cell line, and its angiogenic activity. J Natl Cancer Inst 78:667–673 Ishiwata I, Ishiwata C, Nozawa S, Ishikawa H (1987) Cytological properties of human ovarian carcinoma cell lines. Asia-Oceania J Obstet Gynaecol 13: 79–86 Barnes D (1987) Serum-free animal cell culture. Bio technique 5:534–542 Mackillop WJ, Dotsikas G (1988) Cellular heterogeneity in human epithelial neoplasm. Int J Cell Cloning 6:161–178 Sekiya S, Inaba N, Ishige H, Takamizawa H (1987) In vitro and in vivo studies of the production of placental proteins (hCG, SP1, PPs) in human choriocarcinoma cell lines. Arch Gynecol Obstet 241:111–120 Buick RN (1984) Cell heterogeneity in human ovarian carcinoma. J Cell Physiol [Suppl.] 3:117–112 Author information Authors and Affiliations Rights and permissions About this article Cite this article Sekiya, S., Tanaka, N. & Takamizawa, H. Production of various marker substances in human ovarian cancer cell lines of epithelial origin. Arch Gynecol Obstet 248, 103–110 (1990). https://doi.org/10.1007/BF02389582 Issue date: DOI: https://doi.org/10.1007/BF02389582

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

⚙ Ask this paper AI returns verbatim quotes from the full text · source: oa-doi-fallback ⓘ

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Condition tags

endometriosis

MeSH descriptors

Biomarkers, Tumor Ovarian Neoplasms Tumor Cells, Cultured Adenocarcinoma Adenocarcinoma Antigens, Tumor-Associated, Carbohydrate Antigens, Tumor-Associated, Carbohydrate Biomarkers, Tumor Carcinoembryonic Antigen Carcinoembryonic Antigen Cell Division Cell Division Cell Line Cystadenocarcinoma Cystadenocarcinoma Endometriosis Endometriosis Epithelium Epithelium Female

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.

Source provenance

europepmc
last seen: 2026-09-06T09:34:12.023084+00:00
pubmed
last seen: 2026-05-13T22:12:15.619952+00:00
unpaywall
last seen: 2026-09-07T06:27:18.705824+00:00
License: public-domain-us · commercial use OK · attribution required
Courtesy of the U.S. National Library of Medicine