{"paper_id":"51cf9ca2-fb14-41a1-b835-09736c4b5687","body_text":"Summary\nSome characteristics of cell biology and the production of various tumor markers were examined using 8 human ovarian cancer cell lines of epithelial origin. Structural abnormalities of chromosomes 1, 3, and 6 were relatively common karyotypic changes among the cell lines. Cytoplasmic estradiol or progesterone receptor was not detected in any of the cell lines. A significant heterogeneity of the production of various tumor markers (ferritin, tissue peptide antigen, carcinoembryonic antigen, carbohydrate antigens 125, and 19-9) was noted among the cell lines grown in culture medium supplemented with serum. Three of the 8 cell lines were adapted to proliferate in completely synthetic serum-free culture medium. In addition to marker substances described above, small amounts of progesterone or human chorionic gonadotropin were produced in 2 of the 3 cell lines grown in serum-free culture medium. These results indicate that various marker substances including tumor markers are not produced consistently by human ovarian cancer cells of epithelial origin.\nSimilar content being viewed by others\nReferences\nTorosian MH (1988) The clinical usefulness and limitations of tumor markers. Surg Gynecol Obstet 166:567–579\nJacobs I, Bast RC jr (1989) The CA125 tumor-associated antigen: a review of the literature. Human Reproduct 4:1–12\nYamada T (1974) The cellular biology of a newly established cell line of human ovarian adenocarcinoma in vitro. Keio J Med 23:53–70\nKiyozuka Y, Imai S, Noda T, Morimoto J, Moriyama I, Ichijo M, Tsubura Y (1988) Establishment and characterization of human ovarian cystadenocarcinoma cell line (SHIN-3) which produce CA125 and TPA in vitro. J Jpn Soc Clin Cytol 27:926–935\nIshiwata I, Ishiwata C, Kobayashi N, Ishikawa H (1987) Characterization of newly established cell line HUOA from a human ovarian serous cystadenocarcinoma. Acta Obst Gynaec Jpn 39:831–836\nYamada T, Ueda M (1988) Establishment and characterization of a cell line (OMC-3) originating from human mucinous cystadenocarcinoma of the ovary. Human Cell 1:340–341\nFujii T (1989) Establishment and characterization of human ovarian endometrioid carcinoma cell line. Acta Obst Gynaec Jpn 41:161–166\nIshiwata I, Ishiwata C, Soma M, Ishikawa H (1986) Establishment and characterization of two human ovarian endometrioid carcinoma cell lines (with or without squamous cell component). Gynecol Oncol 25:95–107\nNishida M, Iwasaki H (1988) Establishment of a human ovarian clear cell carcinoma cell line (HAC-2 cells) in vitro. Human Cell 1:345–346\nIshiwata I, Ishiwata C, Soma M, Ishikawa H (1987) Establishment of HUOCA-II, a human ovarian clear cell adenocarcinoma cell line, and its angiogenic activity. J Natl Cancer Inst 78:667–673\nIshiwata I, Ishiwata C, Nozawa S, Ishikawa H (1987) Cytological properties of human ovarian carcinoma cell lines. Asia-Oceania J Obstet Gynaecol 13: 79–86\nBarnes D (1987) Serum-free animal cell culture. Bio technique 5:534–542\nMackillop WJ, Dotsikas G (1988) Cellular heterogeneity in human epithelial neoplasm. Int J Cell Cloning 6:161–178\nSekiya S, Inaba N, Ishige H, Takamizawa H (1987) In vitro and in vivo studies of the production of placental proteins (hCG, SP1, PPs) in human choriocarcinoma cell lines. Arch Gynecol Obstet 241:111–120\nBuick RN (1984) Cell heterogeneity in human ovarian carcinoma. J Cell Physiol [Suppl.] 3:117–112\nAuthor information\nAuthors and Affiliations\nRights and permissions\nAbout this article\nCite this article\nSekiya, S., Tanaka, N. & Takamizawa, H. Production of various marker substances in human ovarian cancer cell lines of epithelial origin. Arch Gynecol Obstet 248, 103–110 (1990). https://doi.org/10.1007/BF02389582\nIssue date:\nDOI: https://doi.org/10.1007/BF02389582","source_license":"public-domain-us","license_restricted":false}