Leukemia inhibitory factor's effect on the growth and survival of sheep's follicles of ovarian tissue during vitrification

One of the experimental programs for fertility protection in women includes protective cryopreservation. Vitroficasion of ovarian tissue is one of the protective cryopreservation methods that use high concentrations of antifreeze and faster cooling. To reduce its complications, LIF (Leukemia inhibitory factor) was used as a pretreatment in this study. In this study, the ovaries were randomly divided into 8 groups. In NCN (without pretreatment and LIF in culture media), NCP (without pretreatment and with LIF in culture media), PCP (with pretreatment and LIF in culture media), and PCN (with pretreatment and without LIF in culture media) groups, vitrification and reversal were not performed. In the groups NVN (without pretreatment and LIF in culture media), NVP (without pretreatment and with LIF in culture media) PV, PVP (with pretreatment and LIF in culture media), and PVN (with pretreatment and without LIF in culture medium) groups, vitrification and tissue reversal were performed. All groups were cultured and histological, cellular, and molecular evaluations were performed. The results of the present study showed that LIF in the culture medium reduced the number of abnormal, primordial, primary, and secondary follicles, and DNA breakage compared to the group without LIF (P < 0.05) and increases the growth of follicles and expression of GDF9, BMP, AMH, KITLG genes (P < 0.05). The use of LIF pretreatment before vitrification and melting of sheep ovary tissue in its culture medium reduces the damage caused by it and increases the growth and development of ovarian follicles while maintaining their function. Similar content being viewed by others Data availability The data supporting this study’s results are accessible from the corresponding author upon judicious demand.

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The authors appreciatively confess the financial backing of the Cellular and Molecular Research Center of Iran University of Medical Sciences. Funding The authors revealed the receiving of the undermentioned financial backing for this article’s research, authorship, and/or publication. The study was backed by the Tehran University of Medical Sciences (25915). Author information Authors and Affiliations Corresponding author Ethics declarations Conflict of interest The author (s) acknowledged no possible conflicts of interest regarding this article’s research, authorship, and /or publication. Ethical approval The Ethics Committee for Animal Experimentation of the Iran University of Medical Sciences permitted the study. Additional information Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Rights and permissions About this article Cite this article Mohammadzadeh, F., Ajdary, M., Mohammadzadeh, A. et al. Leukemia inhibitory factor’s effect on the growth and survival of sheep’s follicles of ovarian tissue during vitrification. Cell Tissue Bank 24, 109–123 (2023). https://doi.org/10.1007/s10561-022-10018-4 Received: Accepted: Published: Version of record: Issue date: DOI: https://doi.org/10.1007/s10561-022-10018-4