{"paper_id":"4f4cf0c3-2a15-4756-b7bd-5d60f1fa31a3","body_text":"Abstract\nOne of the experimental programs for fertility protection in women includes protective cryopreservation. Vitroficasion of ovarian tissue is one of the protective cryopreservation methods that use high concentrations of antifreeze and faster cooling. To reduce its complications, LIF (Leukemia inhibitory factor) was used as a pretreatment in this study. In this study, the ovaries were randomly divided into 8 groups. In NCN (without pretreatment and LIF in culture media), NCP (without pretreatment and with LIF in culture media), PCP (with pretreatment and LIF in culture media), and PCN (with pretreatment and without LIF in culture media) groups, vitrification and reversal were not performed. In the groups NVN (without pretreatment and LIF in culture media), NVP (without pretreatment and with LIF in culture media) PV, PVP (with pretreatment and LIF in culture media), and PVN (with pretreatment and without LIF in culture medium) groups, vitrification and tissue reversal were performed. All groups were cultured and histological, cellular, and molecular evaluations were performed. The results of the present study showed that LIF in the culture medium reduced the number of abnormal, primordial, primary, and secondary follicles, and DNA breakage compared to the group without LIF (P < 0.05) and increases the growth of follicles and expression of GDF9, BMP, AMH, KITLG genes (P < 0.05). The use of LIF pretreatment before vitrification and melting of sheep ovary tissue in its culture medium reduces the damage caused by it and increases the growth and development of ovarian follicles while maintaining their function.\nSimilar content being viewed by others\nData availability\nThe data supporting this study’s results are accessible from the corresponding author upon judicious demand.\nReferences\nAbdi S, Salehnia M, Hosseinkhani S (2016) Quality of oocytes derived from vitrified ovarian follicles cultured in two-and three-dimensional culture system in the presence and absence of kit ligand. Biopreserv Biobank 14(4):279–288\nAdeniran B, Bjarkadottir B, Appeltant R, Lane S, Williams S (2021) Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid. 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The study was backed by the Tehran University of Medical Sciences (25915).\nAuthor information\nAuthors and Affiliations\nCorresponding author\nEthics declarations\nConflict of interest\nThe author (s) acknowledged no possible conflicts of interest regarding this article’s research, authorship, and /or publication.\nEthical approval\nThe Ethics Committee for Animal Experimentation of the Iran University of Medical Sciences permitted the study.\nAdditional information\nPublisher's Note\nSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.\nRights and permissions\nAbout this article\nCite this article\nMohammadzadeh, F., Ajdary, M., Mohammadzadeh, A. et al. Leukemia inhibitory factor’s effect on the growth and survival of sheep’s follicles of ovarian tissue during vitrification. Cell Tissue Bank 24, 109–123 (2023). https://doi.org/10.1007/s10561-022-10018-4\nReceived:\nAccepted:\nPublished:\nVersion of record:\nIssue date:\nDOI: https://doi.org/10.1007/s10561-022-10018-4","source_license":"public-domain-us","license_restricted":false}