The Effects of Endostatin on Ang-2/Tie-2 System in the Transplanted Model of Endometriosis in BALB/C Mice

Objective:To investigate the effects of endostatin on Ang-2/Tie-2 system in ectopic lesion.Methods:Thirty mice of endometriosis model (EM) were established by peritoneum planted.After 1 week of transplantation,model mice were randomly divided into three groups including rAAV2-hEndostatin-EGFP group (n=20),rAAV2-EGFP group (n=20) and PBS control group (n=20).The histology changes were examined in the three groups.The expressions of Ang-2 and Tie-2 were examined by immunohistochemistry.Results:The EM model mice were established successfully.The gland proliferation was obvious in the two control groups.The mesenchymal blood vessel was rich.But there were gland atrophy and blood vessel reducing in endostatin group.There were expressions of Ang-2 and Tie-2 in the cytoplasm of endothelium,epithelial glands and stroma.The positive rate of Ang-2 expression was 40%in endostatin group,much lower than that in other two groups (75% and 80%,P﹤0.05).The positive rate of Tie-2 expression was 45% in endostatin group,which was also lower than that in other two groups (80% and 85%,P0.05).Conclusion:Endostatin can effectively interfere angiogenesis process of Ang-2/Tie-2,which lays the foundation for clinical therapy by anti-angiogenesis.