Immunohistochemical features of benign endometrial hyperplasia in premenopausal women

of the study: to evaluate the immunohistochemical features (phenotypic variants) of benign endometrial hyperplasia in premenopausal women.

and methods. 33 premenopausal women with abnormal uterine bleeding and verified benign endometrial hyperplasia were examined. Expression of the α-receptors for estrogens type 1 (ER1), progesterone receptors and Ki-67 nuclear protein in the endometrium stroma and glands was analyzed. Micromorphometry was performed and the D-score was calculated. Results. Prevalence of comorbid lesions in patients was the combination of endometrial hyperplasia and fibroids (51.4%), cases of abnormal uterine bleedings against submucosal fibroids (13.5%), endometrial polyps (8.1%), combinations of EHP and peritoneal endometriosis (10.8%), adenomyosis and myoma (8.1%), or other combinations of endometrial and myometrial proliferative pathology. D-score for surgery averaged 1.78 ± 0.11 indicating a low risk of malignancy. There were changes after treatment in the quantitative presentation of the studied proteins in stroma and endometrial glands. Thus, before treatment in the glands was determined up to 100% of cells containing ER1 in large quantities, while after treatment their number decreased by an average of 20%. Similar dynamics was observed with progesterone receptors activity. Conclusions. The main prognostic significant phenotypes of endometrial proliferative pathology have been identified. After removing of pathologically chanced endometrium and subsequent treatment with dydrogesterone during 6 months there is prognostically positive decreasing in the ER1 density as well as the Ki-67 protein expressionReferences - Amălinei, C., Păvăleanu, I., Grigoraş, A., et al. “The endometrial regeneration frontiers: from mechanisms to applications in regenerative medicine.” Rom J Morphol Embryol 59.2 (2018): 407–25. - Chiu, H.C., Li, C.J., Yiang, G.T., et al. “Epithelial to Mesenchymal Transition and Cell Biology of Molecular Regulation in Endometrial Carcinogenesis.” J Clin Med 8.4 (2019). - Giuntoli, R.L. 2nd, Gerardi, M.A., Yemelyanova, A.V., et al. “Stage I noninvasive and minimally invasive uterine serous carcinoma: comprehensive staging associated with improved survival.” Int J Gynecol Cancer 22.2 (2014): 273–9. - Jia, M., Sun, P.L., Gao, H. “Uterine lesions with sex cord-like architectures: a systematic review.” Diagn Pathol 14.1 (2019): 129. - Lacheta, J. “Uterine adenomyosis: pathogenesis, diagnostics, symptomatology and treatment.” Ceska Gynekol 84.3 (2019): 240–6. - MacKintosh, M.L., Crosbie, E.J. “Prevention Strategies in Endometrial Carcinoma.” Curr Oncol Rep 20.12 (2018): 101. - Munro, M.G. “Uterine polyps, adenomyosis, leiomyomas, and endometrial receptivity.” Fertil Steril 111.4 (2019): 629–40. - Nijkang, N.P., Anderson, L., Markham, R., Manconi, F. “Endometrial polyps: Pathogenesis, sequelae and treatment.” SAGE Open Med 7 (2019). - Raffone, A., Travaglino, A., Saccone, G., et al. “Should progesterone and estrogen receptors be assessed for predicting the response to conservative treatment of endometrial hyperplasia and cancer? A systematic review and meta-analysis.” Acta Obstet Gynecol Scand 98.8 (2019): 976–87. - Salazar, C.A., Isaacson, K.B. “Office Operative Hysteroscopy: An Update.” J Minim Invasive Gynecol 25.2 (2018): 199–208. - Sanderson, P.A., Critchley, H.O., Williams, A.R., et al. “New concepts for an old problem: the diagnosis of endometrial hyperplasia.” Hum Reprod Update 23.2 (2017): 232–54. - Yang, S., Wang, H., Li, D., Li, M. “Role of Endometrial Autophagy in Physiological and Pathophysiological Processes.” J Cancer 10.15 (2019): 3459–71. - Vannuccini, S., Petraglia, F. “Recent advances in understanding and managing adenomyosis.” F1000Res 8 (2019): F1000 Faculty Rev-283. DOI: 10.12688/f1000research.17242.1 - Bajwa, P., Nielsen, S., Lombard, J.M., et al. “Overactive mTOR signaling leads to endometrial hyperplasia in aged women and mice.” Oncotarget 8.5 (2017): 7265–75. DOI: 10.18632/oncotarget.13919 - Hosseini Nasab, S., Jooya, N., Esmaeili, A., et al. “Using Pyruvate Kinase as a Predictor for Patient With Endometrial Cancer Having Complex Hyperplasia With Atypia to Prevent Hysterectomy and Preserve Fertility: Retrospective Immunohistochemical Study.” Reprod Sci 25.8 (2018): 1286–91. DOI: 10.1177/1933719117741371 - Yin, M., Zhou, H.J., Lin, C., et al. “CD34 Stromal Stem Cells Contribute to Endometrial Regeneration and Repair.” Cell Rep 27.9 (2019): 2709–24.e3. DOI: 10.1016/j.celrep.2019.04.088 - Owuor, T.O., Reid, M., Reschke, L., et al. “Maternal obesogenic diet induces endometrial hyperplasia, an early hallmark of endometrial cancer, in a diethylstilbestrol mouse model.” PLoS One 13.5 (2018): e0186390. DOI: 10.1371/journal.pone.0186390 - Carmina, E., Guastella, E., Longo, R.A. “Advances in the Diagnosis and Treatment of PCOS.” Curr Pharm Des 22.36 (2016): 5508–14. DOI: 10.2174/13816128226661 60719105808 - Kurdoglu, M., Kucukaydin, Z., Kurdoglu, Z., et al. “Expression of Laminin Receptor 1 in Normal, Hyperplastic, and Malignant Endometrium.” Int J Gynecol Pathol 38.4 (2019): 326–34. DOI: 10.1097/PGP.0000000000000535 - Russo, M., Broach, J., Sheldon, K., et al. “Clonal evolution in paired endometrial intraepithelial neoplasia/atypical hyperplasia and endometrioid adenocarcinoma.” Hum Pathol 67 (2017): 69–77. DOI: 10.1016/j.humpath.2017.07.003 - Lauritsen, C.G., Chua, A.L., Nahas, S.J. “Current Treatment Options: Headache Related to Menopause-Diagnosis and Management.” Curr Treat Options Neurol 20.4 (2018): 7. DOI: 10.1007/s11940-018-0492-7 - Bulsa, M., Urasińska, E. “Triple negative endometrial cancer.” Ginekol Pol 88.4 (2017): 212–4. DOI: 10.5603/GP.a2017.0040 - Goldstein, S.R., Lumsden, M.A. “Abnormal uterine bleeding in perimenopause.” Climacteric 20.5 (2017): 414–20. DOI: 10.1080/13697137.2017.1358921 - Bosteels, J., van Wessel, S., Weyers, S., et al. “Hysteroscopy for treating subfertility associated with suspected major uterine cavity abnormalities.” Cochrane Database Syst Rev 12 (2018): CD009461. DOI: 10.1002/14651858.CD009461.pub4 - Gordts, S., Grimbizis, G., Campo, R. “Symptoms and classification of uterine adenomyosis, including the place of hysteroscopy in diagnosis.” Fertil Steril 109.3 (2018): 380–8.e1. DOI: 10.1016/j. fertnstert.2018.01.006 Downloads Published How to Cite Issue Section License Copyright (c) 2020 Н. М. Рожковська, І. С. Ломакіна This work is licensed under a Creative Commons Attribution 4.0 International License. Authors who publish with this journal agree to the following terms: - Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. - Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.